摘要
目的研究槲皮素（ｑｕｅｒｃｅｔｉｎ，ＱＵＥ）对培养人视网膜色素上皮（ｒｅｔｉｎａｌｐｉｇｍｅｎｔｅｐｉｔｈｅｌｉｕｍ，ＲＰＥ）细胞增生和ＤＮＡ合成以及对表皮生长因子（ｅｐｉｄｅｒｍａｌｇｒｏｗｔｈｆａｃｔｏｒ，ＥＧＦ）促ＲＰＥ细胞增生和ＤＮＡ合成作用的影响。方法用细胞计数和３Ｈ－胸腺嘧啶核苷（３Ｈ－ｔｈｙｍｉｄｉｎｅ，３Ｈ－ＴｄＲ）掺入方法，观察不同浓度（２００、１００、５０、１μｍｏｌ／Ｌ）的ＱＵＥ和最大浓度的ＱＵＥ（２００μｍｏｌ／Ｌ）在不同作用时间（２４～１６８小时），分别单独或与ＥＧＦ共同作用时，对ＲＰＥ细胞增生及ＤＮＡ合成的影响；排除着色的死细胞，用活细胞计数法判断药物的细胞毒作用。结果ＱＵＥ２００μｍｏｌ／Ｌ具有最强的抑制效应，４８小时时抑制效应已明显出现，９６小时时抑制达到高峰。与ＱＵＥ单独作用相比，ＱＵＥ对ＥＧＦ的促ＲＰＥ细胞增生效应能产生更强的抑制。ＱＵＥ无细胞毒作用，各实验组细胞活力均在８５．００％以上。结论ＱＵＥ以剂量依赖和时间依赖的方式抑制ＲＰＥ细胞的增生，尤其是由ＥＧＦ刺激的增生。
Objective To investigate the effects of QUE on proliferation and DNA synthesis of cultured retinal pigment epithelium(RPE)cells with or without EGF. Methods With or without EGF,cultured RPE cells were treated with QUE by various concentrations(200,100,50,1 μmol/L)and with QUE 200 μmol/L at different times(24～168 hr),cells proliferation and DNA synthesis were evaluated by cell count method and the uptake of thymidine.The viability of cells was determined by trypanblue exclusion. Results The best concentration of QUE which inhibits proliferation and DNA synthesis of RPE cells was 200 μmol/L.The significant inhibition effect of QUE occurred at 48hr,and the stongest inhibition of QUE occurred at 96 hr.QUE had more powerful effect of antiproliferation on RPE cells,and the viability of RPE cells was over 85.00%. Conclusion The results suggested that QUE could inhibit the proliferation of RPE cells in a dose dependent and time dependent manner,especially inhibit the proliferation induced by EGF stimulating.QUE had no cyto toxic effect on RPE cells cultured in vitro.
出处
《中华眼底病杂志》
CAS
CSCD
北大核心
1999年第1期27-29,共3页
Chinese Journal of Ocular Fundus Diseases
关键词
色素上皮
DNA合成
视网膜病变
槲皮素
Pigment epithelium of eye/drug effects Quercetin Cell count Thymidine nucleotides/administration and dosage Dose response relationship,drug Time factors Cell division/drug effects DNA/biosynthesis
