摘要
目的通过建立HPLC法测定复方新霉素溶液中地塞米松磷酸钠的含量。方法采用高效液相色谱法,色谱柱：kromasil-C18（250mm×4.6mm,5μm）;流动相：甲醇-0.34%磷酸二氢钾溶液（60∶40）;检测波长：242nm;柱温：20℃;流速：0.7mL.min^-1。结果地塞米松磷酸钠的含量在0.4128-2.0640μg范围内线性关系良好。回归方程：Y=2×10^6X-11264,r=0.9999,加样回收率平均值为100.2%。结论本方法快速、结果准确,可用于质量控制。
OBJVECTIVE To establish a HPLC method for determination the content of Dexamethasone Sodium Phosphate in compound Prescirption Neomycin solution.METHODS The high-performance liquid chromatography,Column：kromasil-C18（250mm×4.6mm,5μm）,Mobile phase：methanol0.34% KH2PO4 solution（60∶40）;Detection wavelength：242nm;The temper of oven：20℃;Flow rate：0.7mL·min^-1.Results：the content of Dexamethasone Sodium Phosphate in range of 0.4128～2.0640μg a good linear relationship.The regression equation：Y=2×10^6X-11264,r=0.9999,Recoveries averaged is 100.2%.CONCLUSION The HPLC method is quick and accurate,can be used for the quality control.
出处
《海峡药学》
2010年第10期62-64,共3页
Strait Pharmaceutical Journal
关键词
复方新霉素溶液
地塞米松磷酸钠
HPLC
Compound Prescirption Neomycin solution
Dexamethasone Sodium Phosphate
HPLC
