摘要
茉莉酸(JA)和水杨酸(SA)是植物中重要的内源激素和防御反应信号分子,快速而准确地定量测定植物内源SA和JA含量非常重要。以番茄叶片作为材料,研究利用气相色谱(GC)同时测定植物内源JA和SA的含量。利用丙酮/柠檬酸(50mmol/L)(体积分数=7/3)和乙酸乙酯提取番茄叶片中的JA和SA,提取液经N2吹干后用三甲基硅烷化重氮甲烷甲酯化,然后用顶空-固相微萃取(HS-SPME)方法进行收集,正己烷洗脱后进行气相色谱分析,用氢离子火焰检测器(FID)进行检测,梯度升温程序为60℃(1min)至250℃,15℃/min,250℃,3min。从样品中得到与茉莉酸甲酯(MeJA)和水杨酸甲酯(MeSA)标准品完全吻合的色谱峰。该方法可以广泛用于同时测定植物内源的SA和JA含量。
Jasmonic and salicylic acids are important phytohormones and plant defensive signaling compounds.Here a new method was developed for the simultaneous quantification of the two signaling compounds in tomato leaves by using headspace-solid phase microextraction（HS-SPME） and gas chromatography with hydrogen ion flame detector（FID）.Leaf samples were extracted by mixture of acetone and citric acid（50 mmol/L）（V/V=7/3）,and ethyl acetate.Then the supernatant was dried by N2 and subsequently methylated with trimethylsilyldiazomethane.The volatilized compounds were collected by using headspace-solid phase microextraction on Tenax adsorbents,and eluted with n-hexane.Eluted samples were analyzed by using GC with hydrogen ion flame detector（FID）.The temperature gradient was increased from 60 ℃（1 min） to 250 ℃ in a rate of 15 ℃/min and held on 3 min at 250 ℃.The final chromatographic peaks of JA and SA in the samples were identical to the authentic compounds.This method will be useful for simultaneous quantification of JA and SA in plants.
出处
《江西农业大学学报》
CAS
CSCD
北大核心
2010年第5期1056-1060,共5页
Acta Agriculturae Universitatis Jiangxiensis
基金
国家自然科学基金项目(30870390)
关键词
气相色谱
信号分子
茉莉酸
水杨酸
甲酯化
顶空-固相微萃取
gas chromatograph
signal compounds
jasmonic acid
salicylic acid
methylation
headspace-solid phase microextraction
