补气活血法对兔视网膜静脉阻塞视网膜血管内皮细胞生长因子表达的影响被引量：14

The effect of buqihuoxue(补气活血) therapeutics on expression of retinal vascular endothelial growth factor in the rabbits with experimental retinal vein occlusion

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摘要目的研究补气活血中药治疗视网膜静脉阻塞(retinalveinocclusion,RVO)的作用机理。方法纯种新西兰白兔26只,用倍频激光光凝形成各分支视网膜静脉阻塞的模型,随机分为血栓通组、血栓通加黄芪注射液组、尿激酶组和生理盐水组,治疗观察20天,免疫组化S-P法观察视网膜血管内皮细胞生长因子(vascularendothelialgrowthfactor,VEGF)的表达。结果血栓通组和血栓通加黄芪组VEGF的表达呈弱阳性,尿激酶组呈阳性,生理盐水组呈强阳性。结论补气活血中药的干预可抑制VEGF的表达,达到减少和延缓视网膜新生血管的形成和发展。 OBJECTIVE To prove effect mechanism of the buqihuoxue(补气活血)therapeutics in treatment of retinal vein occlusion METHODS Twenty-six New Zealand rabbits with experimental retinal vein occlusion(RVO)were induced by laser photocoagulation Then the rabbits were divided into random xueshuantong(血栓通)group,xueshuantong and milkvetch root group,Urokinase group and normal saline group All rabbits were put to death and selected their model eyes after curing them for twenty days,detected expression of VEGF by immunohistochemical test S-P RESULTS The express is week positive signal on xueshuantong group,and xueshuantong and milkvetch root group,and positive signal on Urokinase group as well as strong positive signal on normal saline group CONCLUSION Expression of VEGF could be inhibited partially by treatment with Chinese medicine for buqihuoxue It could reduce and defer the formation and development of the retinal neovascularization

作者詹宇坚程慧君余杨桂王燕张淳

机构地区广州中医药大学第一附属医院眼科广州中医药大学

出处《中国中医眼科杂志》 2004年第4期211-213,共3页 China Journal of Chinese Ophthalmology

基金广东省中医药管理局资助课题(B97681)

关键词视网膜静脉阻塞补气活血法血管内皮细胞生长因子 retinal vein occlusion,RVO buqihuoxue(补气活血)therapeutics vascular endothelial growth factor,VEGF

分类号 R285.5 [医药卫生—中药学]

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1Ferrara N, Davis Smyth T.The biology of vascular endothelial growth 5Murata T, Nakagawa K, Khalil A.Ishibashi T, Inomata H, Sueishi K. The temporal and spatial vascular endothelial growth factor expression inretinal vasculogenesis of rat neonates [J] .Lab
2AielloLP, FerraraN, KingGL, etal. Hypoxicregulationandbioactivity of vascular endothelial growth factor: Characterization in retinal microvascular pericytes and pigment epithelial cell [ J] . Invest Ophthalmol Vis Sci, 1994, 35: 1868.
3Leung D W, Cachianes G, Kuang W J, et al. Vascular endothelial growth factor is a secreted and angiogenic mitogen [J] . Science, 1989,246: 1306- 1309.
4Wells J A, Murthy R, Chibber R, et al. Levels of vascular endothelial growth factor are elevated in the vitreous of patients with subretinal neovascularization [J] .BRJOphthalmol, 1996, 80: 363.
5SmithLEH, KopchickJJ, ChenW. etal. Essential role of growth hormone in ischemia - induced retinal neovascularization [ J ] . Science,1997, 276 (13): 1706-1708.
6张悦,宋跃 130041长春.血管内皮生长因子受体与眼新生血管及增生性病变[J].眼科研究,2002,20(2):179-182. 被引量：6
7施新猷.医学动物实验学[M].北京:人民卫生出版社,1980.448.
8杨萍,张惠蓉.视网膜静脉阻塞和Eales病患者玻璃体内皮生长因子含量测定[J].中华眼底病杂志,1997,13(3):171-173. 被引量：7

二级参考文献28

1[1]Ferrara N,Henzel WJ.Biochem Biophys Res Com,1989,161∶851
2[2]Takagi H,King GL,Aiello LP,et al.Diabetes,1996,45∶1016
3[3]Larrivee B,Karsan A.Int J Mol Med,2000,5∶447
4[4]Shinkai A,Ito M,Anazawa H,et al.J Biol Chem,1998,273∶31283
5[5]Fuh G,Li B,Crowley C,et al.J Biol Chem,1998,273∶11197
6[6]Waltenberger J,Claesson-Welsh L,Siegbahn A,et al.J Biol Chem,1994,269∶26988
7[7]Stitt AW,Simpson D,Boocock C,et al.J Pathol,1998,186∶306
8[8]Kim I,Ryan AM,Rohan R,et al.Invest Ophthalmol Vis Sci,1999,40∶2115
9[9]Blaauwgeers HG,Holtkamp GM,Rutten H,et al.Am J Pathol,1999,155∶421
10[10]Simpson DA,Murphy GM,Bhaduri T,et al.Biochem Biophys Res Commun,1999,262∶333