摘要
目的观察去卵巢抑郁症大鼠模型海马5-羟色胺(5-HT)含量及其合成限速酶-色氨酸羟化酶2(TPH2)表达的变化。方法雌性SD大鼠,在卵巢切除术的基础上,联合孤养和21 d慢性不可预见性温和刺激(CUMS)制备去卵巢抑郁症大鼠模型,以行为学测试进行评价。用高效液相色谱-电化学法((HPLC-ECD)测定海马5-HT含量;用RT-PCR技术检测中缝核TPH2 mRNA表达量;用荧光免疫组化检测中缝核TPH2蛋白表达。结果 21 d应激后,模型组大鼠直立活动得分及水平活动得分均减少,糖水消耗百分比下降;海马5-HT含量下降;中缝核TPH2 mRNA表达量及TPH2阳性神经元数量减少。结论在卵巢切除术的基础上,联合孤养和21 d CUMS可造成去卵巢抑郁症大鼠模型;模型大鼠海马5-HT含量下降,可能是中缝核TPH2的表达减少所致。
Objective To identify changes of the concentration of 5-hydroxytryptamine（5-HT） in hippocampus and the expression of tryptophan hydroxylase 2（TPH2） in median raphe nuclei in the ovariectomized depression model of rats.Methods The animal model was established by resecting the ovaries of female rats,then giving isolation-housing in combination with chronic unexpected mild stress（CUMS） for 21 days.Behavioral test was used to evaluate the model.High performance liquid chromatogram-electrochemical detection was employed to measure 5-hydroxytryptamine（5-HT） in hippocampus.Reverse transcription-polymerase chain reaction（RT-PCR） and immunohistochemistry were used to detect the relative expression of TPH2 mRNA and TPH2 immunoreactive neurons in median raphe nuclei.Results After 21 days of CUMS,both crossing and rearing in model group were reduced in Open-Field test,and percentage of sugar consumption was decreased.The concentration of 5-HT in hippocampus was lowered in the model one,the relative expression of TPH2 mRNA and the number of TPH2 immunoreactive neurons in raphe nuclei were decreased too.Conclusion Ovariectomized depression model could successfully be induced by ovariectomy,isolation-housing in combination with 21 days of CUMS.The decrease of 5-HT in hippocampus might be due to the reducing of expression of TPH2 in median raphe nuclei,which leads to lower the synthesis of 5-HT.
出处
《基础医学与临床》
CSCD
北大核心
2010年第11期1167-1171,共5页
Basic and Clinical Medicine
基金
江西省自然科学基金(2007GQY0944)
