摘要
目的重组ＡＰＰ基因真核表达载体。方法采用定向克隆技术、脂质体转染和Ｎｏｒｔｈｅｎｂｌｏｔ杂交技术。结果在分别独立的反应中，ＰＤＧＦ启动子、ＡＰＰｃＤＮＡ、载体依摩尔比３∶３∶１及１∶１∶１同时连接，重组体利用脂质体介导转染ＳＹ５Ｙ神经母细胞瘤细胞，Ｎｏｒｔｈｅｒｎｂｏｌｔ杂交检测到瞬时表达。结论构建具有神经组织特异性的ＡＰＰ基因真核表达载体。
Contemporary research shows that the
mutation overproduction of and accumulation of β amyloid precuser protein(β APP) is closely
related to the developmens of Alzheimer's disease. We constructed the recombinant plasmid
containing the human APP cDNA 695, 751 V717I driven by PDGF promoter which
expressed specifically in nervous system. With two respecting molar ratio of 3∶3∶1 and 1∶
1∶1, the promoter, APP cDNA were subcloned into the vector. The recombinant was introduced
into SY5Y cell by lipeofectamine, and the expression of the construction was analysed by
Northern blot. Now, we are microjecting the transgene into the male pronucleus of the zygotes,
aim at providing a resembling animal model of the human mechanism.
出处
《中国老年学杂志》
CAS
CSCD
北大核心
1999年第4期239-240,共2页
Chinese Journal of Gerontology
基金
国家科技部资助
