摘要
【目的】观察慢性胃炎患者胃黏膜炎症改变和热休克蛋白70(HSP70)、核因子-κB(NF-κB)及其下游炎症因子白细胞介素-8(IL-8)、肿瘤坏死因子-α(TNF-α)蛋白水平的表达,并以脾气虚证为对照,探讨脾胃湿热证与HSP70及NF-κB炎症通路表达的关系。【方法】临床收集慢性胃炎患者51例(包括脾胃湿热证36例、脾气虚证15例),另招募正常志愿者8例作为正常对照组。胃镜下取胃窦黏膜,采用苏木素—伊红(HE)染色检测炎症改变,快速尿素酶和美蓝法检测幽门螺杆菌(Hp)感染,抗生蛋白链菌素—生物素免疫组织化学法检测各组HSP70、NF-κB、IL-8及TNF-α蛋白水平表达。【结果】脾胃湿热证与脾气虚证Hp感染率相当,但脾胃湿热证Hp感染程度及炎症程度均较脾气虚证明显,尤以脾胃湿热证Hp阳性患者炎症程度最重;胃黏膜炎症程度及活动度与Hp感染呈正相关。脾胃湿热证患者HSP70、NF-κB、IL-8及TNF-α表达均较脾气虚证显著升高(P<0.05)。从Hp感染与HSP70及NF-κB炎症通路情况看,脾胃湿热证Hp阳性患者HSP70、NF-κB表达显著高于Hp阴性患者(P<0.05);尽管脾胃湿热证Hp阳性患者IL-8、TNF-α表达与Hp阴性患者之间的差异无显著性意义,但也呈增高趋势。胃黏膜Hp感染与HSP70、NF-κB及IL-8表达呈一定程度的正相关,但与TNF-α表达无明显相关性。【结论】慢性胃炎脾胃湿热证的发生与胃黏膜HSP70及NF-κB炎症通路的表达相关。HSP70与NF-κB及其下游炎症因子在胃黏膜的过表达可能部分体现了慢性胃炎脾胃湿热证"邪正交争"的亢奋状态;相比之下,慢性胃炎脾胃湿热证Hp阳性患者"邪正交争"更为剧烈。
Objective To observe inflammatory changes and expression levels of heat shock protein 70 （HSP70） and nuclear factor-kappa B （NF-κB） as well as the correlated inflammatory factors of interleukin 8 （IL-8） and tumor necrosis factor alpha （TNF-α） in gastric mucosa of chronic gastritis patients, and to explore the relationship of spleen-stomach damp-heat syndrome with HSPT0 and NF-κB inflammatory pathway. Methods Fifty-one chronic gastritis patients were enrolled into the study. Of them, 36 patients were classified into spleen-stomach dampheat syndrome and 15 into spleen-Qi deficiency syndrome. Eight healthy volunteers served as the control. Gastric mucosa was sampled under gastroscope. HE staining was applied for the inflammatory changes of gastric mucosa. Helicobacter pylori （Hp） infection of gastric mucosa was detected by fast urea enzyme and methylene blue method. The protein expression of HSP70, NF-κB, IL-8 and TNF-α in gastric mucosa was detected by Streptavidin-Biotin Complex （SABC） immunohistochemical method. Results The gastric mucosal Hp infection rate in patients with spleen-stomach damp-heat syndrome was similar to that in patients with spleen-Qi syndrome. However, patients with spleen-stomach damp-heat syndrome had severer Hp infection and inflammatory changes than those in patients with spleen-Qi syndrome. The inflammatory changes were more obvious in spleen-stomach damp-heat patients with Hp infection positive. The inflammatory degree and activity of gastric mucosa was positively correlated with Hp infection. The protein expression level of HSP70, NF-κB, IL-8 and TNF-α in gastric mucosa of patients with spleen-stomach damp-heat syndrome was higher than that in patients with spleen-Qi deficiency syndrome （P 〈 0. 05 ）. In regards of relationship of Hp infection with the condition of HSP70 and NF-κB inflammatory pathway, spleen-stomach damp-heat syndrome patients with Hp positive had higher HSP70 and NF-κB expression levels than those with Hp negative （P 〈 0. 05）. The expression of IL-8 and TNF-α showed an increasing trend in spleen-stomach damp-heat syndrome patients with Hp positive as compared with that in Hp negative patients, but the difference was insignificant. Gastric mucosal Hp infection was positively correlated with HSP70, NF-κB and IL-8 expression to some extent, while was not correlated with the expression of TNF-α. Conclusion The occurrence of spleen-stomach damp-heat chronic gastritis is related with HSP70 and NF-κB inflammatory pathway of gastric mucosa. The over-expression of gastric mucosal HSP70 and NF-κB and their associated inflammatory factors maybe partially reflect the excited state of the struggling of healthy Qi or pathogenic Qi in spleen-stomach damp-heat chronic gastritis patients, which is more obvious in those patients with Hp positive.
出处
《广州中医药大学学报》
CAS
2010年第6期587-591,669,670,共7页
Journal of Guangzhou University of Traditional Chinese Medicine
基金
国家自然科学基金资助项目(编号:30772689)
广东省自然科学基金资助项目(编号:7004802)
关键词
慢性胃炎
脾胃湿热
脾气虚
胃黏膜/病理学
CHRONIC GASTRITIS
SPLEEN-STOMACH DAMP-HEAT
SPLEEN-QI DEFICIENCY
GASTRIC MUCOSA/pathology
