摘要
目的:观察恒古骨伤愈合剂干预激素性股骨头坏死兔模型的组织外观和光镜观察改变,并与没有干预的样本之间进行比较。方法:将150只新西兰白兔按随机数字表法分为空白组(A组)24只,正常兔服用恒古骨伤愈合剂对照组(B组)24只,实验组102只。对实验组耳缘静脉注射大肠杆菌内毒素(10μg.kg-1)2次,注射间隔24 h。并于第2次注射大肠杆菌内毒素后,臀肌注射甲强龙(20 mg.kg-1)3次,注射间隔24 h,制备兔激素性股骨头缺血坏死模型。于造模后第5周,将造模成功的53只新西兰白兔以随机数字表法分配其中的48只至模型组(C组,造模后不喂恒古骨伤愈合剂)和恒古骨伤愈合剂治疗组(D组,造模后喂恒古骨伤愈合剂),每组24只。B组和D组以恒古骨伤愈合剂(按经由人与兔体表面积用药量换算计算出的剂量)灌胃,2 d 1次;A组和C组以等体积的蒸馏水灌胃。造模后8,12,16周,截取股骨头行外观观察和电镜观察。结果:外观观察及光镜观察显示C组出现明显的骨坏死灶,骨髓腔内可见大量脂肪细胞增生;与C组比较,D组骨细胞受损程度较轻,骨小梁密度较高,空骨陷窝比率较少,骨坏死面积和髓腔内脂肪细胞数量明显减少,骨坏死修复迹象明显;A组与B组股骨头形态正常。结论:恒古骨伤愈合剂可有效促进早期激素性股骨头坏死兔模型坏死股骨头的修复。
Objective： To evaluate the histological changes on the femoral heads of the SANFH rabbit animal models and after it were intervened by Osteoking（herbs of the Yi minority in Yunnan province） using general and light microscope observation.Method： A total of 150 New Zealand white rabbits were randomly divided into a non-treatment control group（A group,n=24）,normal rabbits with Osteoking treatment group（B group,n=24）,and the experimental group（n=102）.The experimental group was injected with escherichia coli endotoxin（10 μg·kg^-1） into auricular vein twice by 24-hour intervals,and prednisolone（20 mg·kg^-1） was injected into buttock three times by 24-hour intervals to make steroid-induced femoral head necrosis model.At the fifth week,48 out of 53 rabbits were equally divided into model group（C group,n=24,models with non-treatment Osteoking） and abnormal rabbits with Osteoking treatment group（D group,n=24）.B group and D group were intragastrically administrated with Osteoking,once every two days.A group and C group were intragastrically administrated with the equal volume of saline.At 8th,12th and 16th week after model preparation,the femoral head specimens were observed under the general and a light microscope.Result： Macroscopic and light microscopic analysis showed that,clear bone necrosis of femoral head was observed in the C group,and a large number of fat cell proliferation was found in the bone marrow cavity.As compared with C group,the damage level of cells in D group was milder,however,the density of bone trabecula from Osteoking treatment was high,and the ratio of bone lacuna was very low.It is also demonstrated that the surface area of bone necrosis was decreased,and the number of cells from adiposities was reduced significantly.The phenomenon of bone necrosis repaired apparently.The morphology of femoral head from A group and B group is normal.Conclusion： It suggested that Osteoking could effectively help repair steroid-induced femoral head necrosis in the early stage.
出处
《中国中药杂志》
CAS
CSCD
北大核心
2010年第21期2912-2916,共5页
China Journal of Chinese Materia Medica
基金
国家自然科学基金项目(81060361
30460162)
云南省自然科学基金项目(2010ZC169)
云南省教育厅基金项目(2010C212)
