摘要
目的:探讨川芎嗪与葛根素合用对糖氧剥夺诱导的体外血脑屏障(aBBB)通透性的影响。方法:实验分为正常对照组、模型组、川芎嗪组、葛根素组、葛根素与川芎嗪合用组和尼莫地平组6个组。原代培养大鼠脑微血管内皮细胞和星形胶质细胞共培养构建aBBB模型,经糖氧剥夺诱导处理建立aBBB损伤模型,跨内皮电阻抗、γ-谷氨酰转肽酶、碱性磷酸酶及乳酸脱氢酶鉴定aBBB损伤情况,高效液相色谱法测定川芎嗪、葛根素及尼莫地平的含量,并计算在OGD诱导下的aBBB药物透过浓度。结果:与对照组相比,模型组TEER值降低(P<0.01),川芎嗪组、川芎嗪与葛根素合用组、尼莫地平组-γGT,AKP升高(P<0.01),与川芎嗪与葛根素合用组相比,川芎嗪、葛根素单用AKP升高明显(P<0.01),尼莫地平各指标均有显著性差异(P<0.05),提示川芎嗪与葛根素合用在可升高TEER,增加-γGT,AKP含量和降低LDH具有协同作用,OGD诱导下aBBB模型的培液中川芎嗪与葛根素合用组各成分透过浓度均较川芎嗪、葛根素单用增加。结论:川芎嗪与葛根素合用较两者单用易透过aBBB,且对aBBB有一定的保护作用,可能与降低OGD诱导下的aBBB通透性有关。
Objective： To explore permeability of artificial blood-brain barrier（aBBB） by oxygen-glucose deprivation combined（OGD）-induced using tetramethylpyrazine combined with puerarin in vitro.Method： Rats were divided into normal control group,model group,tetramethylpyrazine group,puerarin group,tetramethylpyrazine-puerarin group and nimodipine group.Culture rat brain microvascular endothelial cells and astrocytes in vitro and build the OGD-induced aBBB damage model.Evaluate aBBB damage characteristics by TEER,γ-GT,AKP and LDH.Determine contents of tetramethylpyrazine,puerarin,nimodipine and calculate drug permeating concentration of OGD-induced aBBB model by HPLC.Result： Compared with the model,the level of TEER was lower than the control group with significant difference（P〈0.01）.The levels of γ-GT,AKP in tetramethylpyrazine group,tetramethylpyrazine-puerarin group and nimodipine group were higher than the model group,the differences were significant（P〈0.01）.Compared with tetramethylpyrazine group or puerarin group,the level of AKP of tetramethylpyrazine-puerarin group increased significantly（P〈0.01）.The differences of levels of TEER,γ-GT,AKP and LDH between tetramethylpyrazine-puerarin group and nimodipinthe group were significant（P〈0.05）.Tetramethylpyrazine-puerarin group has a synergistic effect of increasing TEER,γ-GT,AKP and reducing LDH.The permeating rate in tetramethylpyrazine-puerarin group was higher than tetramethylpyrazine group and puerarin group.Conclusion： Tetramethylpyrazine-puerarin can permeate aBBB more easily and protect aBBB.The cause may relate to reducing the permeability of the OGD-induced aBBB.
出处
《中国中药杂志》
CAS
CSCD
北大核心
2010年第20期2745-2748,共4页
China Journal of Chinese Materia Medica
基金
国家自然科学基金项目(30873430
30973933)
关键词
川芎嗪
葛根素
体外血脑屏障
通透性
puerarin
tetramethylpyrazine
artificia/blood brain-barrier
permeability
