摘要
报道了流感病毒体外诱导狗肾细胞系（ＭＤＣＫ）细胞凋亡的检测结果，对黑色素选择性抑制流感病毒诱导细胞凋亡的可能性进行了探讨，同时与临床上常用的抗病毒药物病毒唑的效果进行比较。结果显示：病毒感染６ｈ后，即可观测到宿主细胞核固缩现象、ＤＮＡ凝胶电泳出现特征性的梯状图谱，感染１２ｈ后，细胞核可见明显的裂解；并且流感病毒株Ａ１／京防８６１诱导细胞凋亡能力强于Ｂ沪防／９３－１；在２０～１２５μｇ／ｍＬ浓度范围内，黑色素可有效抑制６４个血凝单位（ＨＵ）的流感病毒感染诱导的细胞凋亡而无细胞毒性作用，其抑制效率类似病毒唑。
The apoptosis induced by influenza virus in cultured MDCK cells was reported and the selective inhibitory effect of melamin on the apoptosis induced by influenza virus was investigated. The results showed that the DNA ladder could be first detected at 6 h post infection (p.i.), accompanied by nuclear condensation and nuclear fragmentation could be easily detected at 12 h p.i. In addition, the apoptosis induced activity of influenza virus A1/Jingfang 86 1 strain was more potent than that of B/Hufang 93 1 strain (P<0.05). In the range of 20-125 μg/mL, melanin was found to significantly (P<0.001) inhibit apoptosis induced by 64 hemagglutination unit influenza virus infection with a inhibitory rate comparable to that obtained by virazole and showed no cytotoxicity. The inital results suggested that the mechanism of melanin against the apoptosis induced by influenza virus was related to the blockage of viruses' adsorbtion to the host cells.
出处
《中国病毒学》
CSCD
1999年第2期140-146,共7页
Virologica Sinica
