摘要
癌症转移是癌症病人死亡的主要原因之一。抑制肿瘤转移的基因ｎｍ２３－Ｈ１与ｎｍ２３－Ｈ２分别编码二磷酸核苷激酶（ＮＤＰＫ）的Ａ与Ｂ亚基〔１〕，ｎｍ２３－Ｈ１基因能有效地抑制肿瘤转移〔２〕。ｎｍ２３－Ｈ１的表达水平与黑色素瘤〔２，３〕、乳腺癌〔４〕、卵巢...
The human tumor suppressor
gene nm23-H1 was cloned into retroviral vector pLXSN and constructed an expressing vector
pLXSN-nmh1. This recombinant plasmid was transfected into ψCRIP paking cell line by
calcium phosphate precipitation technique. The colonies capable of secreting product of
nm23-H1 gene and the recombinant retroviruses were selected by G418. The integration and
expression of nm23-H1 gene were detected by PCR and Western blot hybrization
respectively. This study demonstrates a crucial role of retroviruses as gene therapy and
transgenosis.
出处
《病毒学报》
CAS
CSCD
北大核心
1999年第2期185-187,共3页
Chinese Journal of Virology
