摘要
目的：为了建立一种供临床实验室广泛应用的检测单核／巨噬细胞ＡＤＣＣ效应的实验方法。方法：选择ＣＴＬＬ２细胞作为靶细胞，制备出兔抗ＣＴＬＬ２细胞特异性抗血清，用单核细胞作为效应细胞，通过ＣＴＬＬ２细胞增殖结果检测单核细胞ＡＤＣＣ效应，并与传统检测方法１２５ＩＵｄＲ释放试验进行比较。结果：该方法较同位素试验方法更敏感。结论：细胞增殖法检测单核细胞ＡＤＣＣ效应准确、可靠。
Objective:To create a method of detecting the ADCC effect of monocyte/macrophage in clinical
laboratory.Methods:CTLL2 cells was selected as target cells to prepare the specific antiserum
of rabbit.Monocytes act as effect cells.The ADCC effect of monocyte was detected by observing
the proliferation of CTLL2 cells.This new method was also compared with the traditional method
of 125 I UdR releasing test.Results:The method of cell proliferation is more sensitive than
that of isotope releasing test.Conclusion:Using cell proliferation method to detect the ADCC
effect of monocyte is stable and accurate.
出处
《中国免疫学杂志》
CAS
CSCD
北大核心
1999年第4期177-179,共3页
Chinese Journal of Immunology
