摘要
【目的】采用ISSR技术从DNA水平探讨核桃材料亲缘关系,为核桃资源的保护、开发利用及新品种选育等提供理论依据。【方法】以核桃叶片为材料,利用ISSR标记技术,筛选出8条引物对24份DNA进行PCR扩增。以NTSYSpc2.1软件计算样品的遗传相似系数(GS),并进行UPGMA聚类分析。【结果】共扩增位点86个,其中多态位点60个,多态位点百分率70.00%;样品间的GS在0.29～0.93,在GS=0.37处将栽培核桃与野生核桃分成2组。【结论】供试核桃具有较高的多态性,样品间有明显的基因分化,其亲缘关系也较远,并且具有明显的地域性。
【Objective】To analyse the genetic relationship of walnuts at DNA level by ISSR technique.The findings of this research would provide theoretic evidence for the protection of walnut resources,its development and utilization and selective breeding of new varieties.【Method】ISSR analysis of samples was conducted with new-born leaf as material,8 ISSR primers were screened and conducted PCR amplification for 24 DNA.The genetic similarity（GS） of the samples were calculated by NTSYSpc2.1 software,and UPGMA cluster analysis was conducted.【Result】The results showed that 86 loci were detected with the 8 primers,among which there were 60 polymorphic loci,the samples have polymorphic ratio of 70.00%.The genetic similarity（GS） among 24 walnuts ranged from 0.29 to 0.93.When GS was 0.37,cultivated walnut species and other nature walnut species could be clustered into two groups.【Conclusion】The study showed that the walnuts tested had high polymorphism,with apparent gene differentiation among samples,relatively far genetic relationship and obvious regional features.
出处
《新疆农业科学》
CAS
CSCD
北大核心
2010年第9期1722-1727,共6页
Xinjiang Agricultural Sciences
基金
国家"十一五"科技支撑项目(2007BAD36B01-3)
新疆维吾尔自治区重大科技专项项目(200731136)
关键词
核桃
ISSR
亲缘关系
walnut（Juglans regia L.）
ISSR
genetic relationship
