摘要
目的本实验以１０ｕｓ／ｄ上皮生长因子（ＥＧＦ）皮下给药促进大白鼠小肠缺血再灌注损伤后粘膜的修复，观察细胞的增殖。方法ＳＤ雄性大白鼠６０只，阻断肠系膜上动脉后去除阻断为小肠缺血再灌注损伤模型，随机分为对照组（Ａ）、皮下放置不含ＥＧＦ缓释片组（Ｂ）和放置ＥＧＦ缓释片组（Ｃ）。观察各组０时（缺血６０分钟再灌注１２０分钟后）、第２、４、８天组织学改变，并进行粘膜细胞ＤＮＡ流式细胞仪分析。结果组织学检查示Ｂ组再生绒毛较Ｃ组短小、稀少。肠粘膜细胞经流式细胞仪ＤＮＡ分析，结果表明４８小时修复，Ｃ组Ｓ期细胞（１３．０２％±１．３９％）较Ｂ组升高更为明显（ＣｖｓＢＰ＜０．０５），损伤修复后第４、８天，Ｂ组细胞增殖已趋于正常，与Ａ组比较Ｐ＞０．０５，而Ｃ组上皮细胞增殖仍居高不下，较Ｂ组和Ａ组均有统计学意义（Ｐ＜０．０１）。结论ＥＧＦ不仅可促进缺血再灌注后小肠粘膜的再生修复，同时有助于消化吸收功能的早期恢复。
Objective To evaluate the influence of long-term continuous administration of epidermalgrowth factor (EGF) on mucosal cell proliferation after intestinal ischemia-reperfusion (I/R) injury. Methods Sixty male SD rats weighting 200-250 grams were divided into equal three groups: Group A (controls) received laparotory only: Group B underwent 60 minutes of SMA occlusion with EGF placebo pelletsimplanted subcutaneously and Group C SMA occlusion and administration of EGF. The bowels were examined for histology and isolating mucosal cells for DNA analysis by flow cytometry. Results intestinal seg-ments showed epithelial denudation and villi impairment after 60 minutes of ischemia and 2 hours withreperfusion in bob I/R groups. The mucosa of all specimens healed with reepithelialized villus tips, but thevilli were scarce and shorter in group B. In the DNA profile the percent of S-phase cells in group B and Cwere higher than that of the control (P<0. 01). At 2 days regeneration, it kept a higher level in group B(B vs A P<0. 01). The S-phase cells of group C (13. 02% 1. 39% ) was the highest in the meantime (Cvs B P<0. 05). At the 4-8 days pened, it still kept a significantly higher level than that of group B. Conclusions Subcutaneous administration of EGF (10 Ug/d) can stimulate crypt cell proliferation, mucosal regeneration and intestinal fraction recuperation.
出处
《中华小儿外科杂志》
CSCD
1999年第3期167-169,共3页
Chinese Journal of Pediatric Surgery
基金
上海市曙光计划
上海市百人计划资助课题
关键词
肠缺血
再灌注损伤
上皮生长因子
修复
Epidermal growth factor ,Intestine small, Mucous membrane
