摘要
利用同源克隆方法首次从草莓花芽cDNA中分离出AP ETALA 2同源基因SAP 2。SAP 2全长1 820 bp,编码435个氨基酸。序列分析表明,SAP 2具有AP 2家族典型的结构域。采用RT-PCR方法分析SAP 2在不同组织中的表达情况,结果显示SAP 2在草莓营养组织、花芽以及不同花器官中均有表达,与拟南芥AP 2、矮牵牛P hAP 2A的表达模式一致。以上结果说明SAP 2是草莓的AP 2同源基因。
SAP2, an APETALA2 homologous gene, was first isolated from the flower buds of strawberry by homologous cloning. Its full length was 1 820 bp and encoded 435 amino acids. Its sequencing indicated that it had the typical domains of AP2 family. The RT-PCR analysis of its expression in different tissues revealed that it expressed itself in the vegetative tissues, flower buds and other different organs and its expression model was consistent with the AP2 expression model of Arabidopsis and the PhAP2A expression models of Petunia. These showed that it is the homologous gene of SAP2 in strawberry.
出处
《西北植物学报》
CAS
CSCD
北大核心
2005年第10期1937-1942,共6页
Acta Botanica Boreali-Occidentalia Sinica
基金
国家自然科学基金(3037014330270651)
烟台师范学院校基金项目(043302)
