摘要
目的研究地塞米松（ＤＭ）及甘草酸（ＧＬ）对小鼠肝细胞凋亡的影响。方法以肝／体重比、肝组织ＤＮＡ含量、组织学改变及原位凋亡细胞ＴＵＮＥＬ反应为指标，观察ＤＭ（９ｍｇ·ｋｇ－１）及ＧＬ（５０ｍｇ·ｋｇ－１），ｉｐ，每８ｈ１次，对撤除苯巴比妥（ＰＢ）后引起的小鼠肝细胞凋亡的影响。结果与ＰＢ不撤除对照组相比，ＤＭ组小鼠肝／体重比及肝ＤＮＡ含量无明显回落，组织学检查未见明显凋亡改变，ＴＵＮＥＬ反应阴性。ＧＬ组小鼠肝／体重比及ＤＮＡ含量分别回落１２０％和１３６％，肝细胞出现典型凋亡改变，ＴＵＮＥＬ反应阳性。结论ＤＭ能阻断撤除ＰＢ诱导的小鼠肝细胞凋亡，甘草酸对其无影响。
AIM To investigate the effects of dexamethasone (DM) and glycyrrhizin (GL) on the hepatocyte apoptosis caused by phenobarbital (PB) withdrawal in mice. METHODS The ratio of liver/body weight, hepatic DNA content, morphological changes and TUNEL reaction (TdTmediated XdUTP nick labeling) as indices of evaluating hepatocyte apoptosis, were used to observe the effects of DM (9 mgkg-1, ip) and GL (50 mgkg-1, ip) on the hepatocyte apoptosis caused by PB withdrawal. RESUITS The regression of hepatic DNA content and ratio of liver/body weight and typical hepatocyte apoptosis, which occured in the control as mice after stopping PB treatment, were not found in the mice treated with DM, and a negative result of TUNEL assay was presented. However, in the mice given GL the ratio of liver/body weight and hepatic DNA content regressed by 120% and 136%, respectively; with a positive reaction of TUNEL assay and the typical changes of hepatocyte apoptosis. CONCLUSION The results showed that DM, but not GL, preve
出处
《中国药理学通报》
CAS
CSCD
北大核心
1999年第2期143-145,共3页
Chinese Pharmacological Bulletin
基金
国家自然科学基金
关键词
地塞米松
甘草酸
苯巴比妥钠
肝细胞凋亡
肝疾病
dexamethasone
glycyrrhizin
phenobarbital
hepatocyte apoptosisnted hepatocyte apoptosis caused by PB withdrawal in mice.
