摘要
目的:探讨高原地区严重烫伤延迟复苏大鼠肺组织中NF-κB的变化规律及其意义。方法:雄性Wistar大鼠240只,分别在1517m和3848m两个海拔高度随机分为延迟复苏组(DFR,n=50)、即时复苏组(IFR,n=60)和对照组(CG,n=10),建立30%Ⅲ度烫伤模型,分别于伤后1、6、12、24、72和168h取材,观察肺组织病理学改变及NF-κB的表达。结果:随着海拔高度增加,肺组织病理变化越明显,且DFR组较IFR组差异显著。NF-κB阳性表达位于肺泡上皮细胞核或胞浆,高海拔组表达强度高于低海拔组,实验组高于对照组,各海拔高度DFR高于IFR组(P<0.05)。结论:NF-κB表达强度变化与高原地区严重烫伤延迟复苏大鼠肺组织损伤有关。
Objective：To explore the expression of NF-κB in lung tissue of rats after severe burn with delayed fluid resuscitation at different altitudes.Methods：A total of 240 male Wistar rats were employed to establish the experimental burn models（30%TBSA,Ⅲdegree）at different altitudes,and they were randomly divided into 3 groups：delayed fluid resuscitation group（DFR,n=50）,immediate fluid resuscitation group（IFR,n=60）and control group（CG,n=10）.The lung samples were harvested at 1,6,12,24,72 and 168 h after burn respectively.Pathological changes and NF-κB protein expression were observed.Results：With the altitude height rising,pathological changes of lung tissue appeared more obvious.The positive expression of NF-κB existed in the nucleus and cytoplasm.The expression of NF-κB was much higher in DFR and IFR than that in CG（P0.05）,much higher in DFR than that in IFR（P0.05）,and much higher at 3 840 m altitude than that at 1 517 m（P0.05）.Conclusion：The NF-κB might contribute significantly to lung injury of severe burned rats with delayed fluid resuscitation at high altitude.
出处
《西北国防医学杂志》
CAS
2010年第5期321-323,共3页
Medical Journal of National Defending Forces in Northwest China
基金
全军医学科学技术研究"十一五"计划课题资助项目(06G030)
关键词
烫伤
延迟复苏
肺损伤
核因子-ΚB
高原
大鼠
Burns
Delayed fluid resuscitation
Lung injury
NF-κB
High altitude
Rat
