摘要
通过在52周龄种母鸡日粮中添加0 mg/kg、0.3 mg/kg、0.6 mg/kg酵母硒(含硒0.1%)和3.2 g/kg、4.0g/kg、5.4 g/kg DL-蛋氨酸(含蛋氨酸99%),测定种蛋中硒含量、谷胱甘肽过氧化物酶(GPx)活力、谷胱甘肽(GSH)含量、脂质过氧化物(MDA)含量和蛋白羰基值来研究硒和蛋氨酸补充量对种蛋抗氧化性的影响。结果表明:①蛋黄中硒含量随着硒补充量增加而极显著增加(P<0.01),3.2 g/kg蛋氨酸处理组蛋黄中硒含量极显著高于4.0g/kg和5.4 g/kg处理组(P<0.01)。②与0 mg/kg和0.6 mg/kg硒处理相比,0.3 mg/kg硒处理组能极显著提高蛋黄中GPx活力(P<0.01),极显著降低蛋黄中MDA含量(P<0.01)。③3.2 g/kg蛋氨酸处理组蛋黄中GPx活力极显著高于4.0 g/kg和5.4 g/kg处理组(P<0.01),3.2 g/kg和4.0 g/kg蛋氨酸处理组蛋黄中GSH含量显著高于5.4g/kg处理组(P<0.05),而蛋黄中MDA含量随着蛋氨酸补充量增加而极显著降低(P<0.01)。④蛋清中GPx活力随着硒补充量增加而显著增加(P<0.05),随着蛋氨酸补充量增加而极显著降低(P<0.01)。⑤5.4 g/kg蛋氨酸处理组蛋清中GSH含量极显著高于3.2 g/kg和4.0 g/kg处理组(P<0.01),3.2 g/kg蛋氨酸处理组蛋白羰基含量极显著高于4.0 g/kg和5.4 g/kg处理组(P<0.01)。由此可见,在种母鸡日粮中适量添加硒和蛋氨酸能够增强种蛋的抗氧化性。
This experiment was conducted to study the effects of selenium（Se） and methionine（Met） supplemented in diets for breeding hens on the contents of Se,glutathione（GSH）,malondialdehyde（MDA） and carbonyl and glutathione peroxidase（GPx） activity in breeding eggs.Four hundred and fifty hens were randomly divided into 9 treatments with 5 replicates each treatment and were fed with corn soybean diets with 0 mg/kg,0.3 mg/kg and 0.6 mg/kg Se-yeast and 3.2 g/kg,4.0 g/kg and 5.4 g/kg DL-methionine,respectively.Twenty eggs per treatment were randomly selected and tested at 0 d,28 d and 56 d of the test period,respectively.The yolk Se concentration significantly increased with the increase of Se supplementation（P〈0.01）,and the yolk Se concentration in 3.2 g/kg Met treatment was higher than those in 4.0 g/kg Met and 5.4 g/kg Met treatments（P〈0.01）.The yolk GPx activity in 0.3 mg/kg Se treatment was significantly higher than those of 0 mg/kg Se and 0.60 mg/kg Se treatments,while the yolk MDA content was lower（P〈0.01）.The 3.2 g/kg Met and 4.0 g/kg Met treatments enhanced the yolk GSH content compared with that of 5.4 g/kg Met treatment,and the yolk GPx activity in 3.2 g/kg Met treatment was significantly higher than those in 4.0 g/kg Met and 5.4 g/kg Met treatments（P〈0.01）.The yolk MDA content significantly decreased with increase of Met supplementation（P〈0.01）.The albumen GPx activity significantly improved with the increase of Se supplementation（P〈0.01）,and decreased with the increase of Met supplementation（P〈0.01）.The albumen GSH content of 5.4 g/kg Met treatment significantly increased compared with those of 3.2 g/kg Met and 4.0 g/kg Met treatments（P〈0.01）,and the albumen carbonyl content in 3.2 g/kg Met treatment was significantly higher than those in 4.0 g/kg Met and 5.4 g/kg Met treatments（P〈0.01）.The conclusion was drawn that Se and Met supplemented in diets for breeding hens could enhance the antioxidant activity of breeding eggs.
出处
《江苏农业学报》
CSCD
北大核心
2010年第5期1003-1008,共6页
Jiangsu Journal of Agricultural Sciences
基金
“973”国家重点基础研究发展计划项目(2004CB117505)
关键词
母体效应
硒
蛋氨酸
抗氧化性
selenium
methionine
antioxidative activity
