摘要
目的了解我国重型肝炎Ｃ基因调节序列变异特点。方法克隆及聚合酶链反应（ＰＣＲ）产物直接测序方法对，检测７例重型乙型肝炎患者乙型肝炎病毒（ＨＢＶ）Ｃ基因调节序列变异。结果４例存在Ｔ１７６２Ａ１７６４变异；但在Ｔ１７６２Ａ１７６４以外位点也存有诸多变异，其中１例在ｎｔ１７７６－１７７７之间有１１ｂｐ的插入变异。结论重型肝炎ＨＢＶＣ基因启动子区存在较多的变异位点，推测这些变异对Ｃ启动子的调节活性影响可能是综合性的。
Objective To characterize mutations in core regulatory sequences of hepatitis B virus in Chinese patients with fulminant hepatitis.Methods Seven patients with fulminant hepatitis were investigated for mutations in core gene regulatory sequences of hepatitis B virus using PCR products and cloning sequencing methods.Results Four in the seven patients had T 1762 A 1764 mutation.Furthermore,there were a lot of variants at other sites in this promoter region,induding one case with a 11bp insert between nt 1776 1777.Conclusion It indicated that there were quite a few mutation sites in the region of core promoter,in the patients with fulmiannt hepatitis and those mutations may effect the activity of core promoter synthetically.
出处
《中华传染病杂志》
CAS
CSCD
北大核心
1999年第2期108-110,共3页
Chinese Journal of Infectious Diseases
基金
国家自然科学九五重点课题基金
