摘要
目的探讨乙型肝炎病毒(HBV)DNAC基因基本核心启动子(BCP)变异对机体免疫状态及乙型肝炎病毒复制的影响。方法采用PCR微板核酸杂交结合ELISA技术检测HBV DNA BCP变异;采用双抗体夹心ELISA法检测患者血清IL-2、IFN-γ、IL-4和IL-10水平;采用荧光定量PCR法测定HBV DNA水平。结果HBV DNA BCP变异病人和非变异病人血清IL-2水平分别为61.4±24.7ng/L和65.1±25.3ng/L,IFN-γ为82.0±50.1ng/L和71.8±67.0ng/L,IL-4为62.3±46.0ng/L和59.4±51.0ng/L,IL-10为74.0±88.2ng/L和81.4±67.0ng/L(P均>0.1);HBV DNA BCP变异病人的HBV DNA水平为1×105.82±2.04copies/ml,明显高于非变异病人的1×104.71±1.28copies/ml(P<0.01)。结论HBV DNA BCP变异对机体血清细胞因子水平无明显影响,但对HBV DNA的复制具有一定的促进作用。
Objective To probe the influence of HBV DNA basal core promoter mutation on serum cytokines and HBV DNA levels in patients with chronic hepatitis B virus infection. Methods Microplate hybridization-ELISA and Fluorescence Quantitative PCR technology were applied to detect HBV DNA BCP mutation and HBV DNA respectively. The level of IL-2,IFN-7,IL-4 and IL-10 in sera was investigated by ELISA. Results The IL-2 levels in patients with and with- out BCP mutation were 61.4±24.7ng/L and 65.1±25.3ng/L; IFN-γ were 82.0±50.1ng/L and 71.8±67.0ng/L; IL-4 were 62.3±46.0ng/L and 59.4±51.0ng/L; and IL-10 were 74.0±88.2ng/L and 81.4±67.0ng/L, respectively （P〉 0.1 ）. The serum HBV DNA levels in HBV DNA BCP mutation group（ 1 × 10^5.82±2.08 copies/ml） was higher than that（ 1 × 10^4.71±1.28 copies/ml） in the non-mutation groups（P〉0.01）. Conclusion The HBV DNA BCP mutation might not change the serum cytokine, but enhance the HBV replication.
出处
《实用肝脏病杂志》
CAS
2008年第1期34-36,共3页
Journal of Practical Hepatology
