摘要
采用薄层扫描法测定黑骨藤药材中β-香树酯醇,并评价不同产地黑骨藤药材的质量。方法中以石油醚-氯仿（1＋1.5）二元溶剂体系为展开剂,在硅胶薄层板上分离得清晰斑点,然后在536 nm波长处采用单波长线性透射扫描斑点。β-香树酯醇的质量在0.39~3.51μg范围内与其峰面积呈线性关系。在0.978,1.220,1.464μg 3个添加水平下β-香树酯醇的平均回收率分别为101.3%,96.8%,100.2%。
Thin-layer chromatographic scanning（TLCS） was used to determine the content of β-amyrin in Periploca Forrestii Schltr.and to evaluate the quality of this medicine from different origins.The plate adsorbed with a thin layer of silica gel was used as the stationary phase,and mixture of petroleum ether and chloroform（mixed in the ratio of 2 to 3 by vol.） was used both as the mobile phase and as developing solvent.The spot of β-amyrin was separated clearly and determined by single-wavelength linear transmission scanning（SWLTS） at 536 nm.Linear relationship between values of peak area and mass of β-amyrin was kept in the range of 0.39-3.51 μg.Test for recovery was made by standard addition method at 3 mass levels of 0.978,1.220,1.464 μg,and the average values of recovery obtained were 101.3%,96.8%,100.2%,respectively.
出处
《理化检验（化学分册）》
CAS
CSCD
北大核心
2010年第10期1179-1181,共3页
Physical Testing and Chemical Analysis(Part B:Chemical Analysis)
基金
贵州省中药现代化科技产业研究开发专项项目[黔科合中药专字[2006]5007号]
贵州省科技计划项目(黔科合带帽字[2008]5005号)
