摘要
Ca2+作为第二信使参与了植物生长和发育过程的调控,不同生物和非生物胁迫信号均可诱导胞内Ca2+变化。对Ca2+在信号转导作用中的认识主要来自于细胞内Ca2+浓度测定。水母发光蛋白和基于荧光蛋白的Ca2+荧光指示剂作为检测细胞Ca2+信号的手段是近年发展起来的新方法。本文综述了水母发光蛋白和基于荧光蛋白的Ca2+荧光指示剂的发展、测量原理、优点与不足及其在细胞Ca2+信号转导中的应用研究进展。
Accumulating evidence suggests that Ca2＋ serves as a messenger in many normal growth and developmental process and in plant responses to biotic and abiotic stresses.Assessment of the role of Ca2＋ in signal transduction requires knowledge of its intracellular levels.As new calibration Ca2＋ methods aequorin and fluorescent protein based Ca2＋ indicators were developed in the recent years.In this review we show the development of Ca2＋ fluorescent probes,the mechanisms of measuring Ca2＋,advantages and disadvantages of aequorin,fluorescent protein based Ca2＋ probes.We also show the progress in cellular Ca2＋ signal research.
出处
《西北植物学报》
CAS
CSCD
北大核心
2010年第10期2134-2140,共7页
Acta Botanica Boreali-Occidentalia Sinica
基金
国家自然科学基金(30800075)
河南大学科研基金(2009YBZR012)
