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基于水母发光蛋白的低温胁迫下拟南芥胞内Ca^(2+)信号转导研究 被引量：3

Study on the Cytoplasmic Calcium Signal Transduction under Low Temperature Based on Aequorin in Arabidopsis thaliana
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摘要 通过花序浸染法获得了转化有水母发光蛋白基因的拟南芥植株,利用该材料研究了低温胁迫下植物细胞内Ca2+浓度的变化.结果表明,低温刺激可引起细胞内Ca2+浓度升高,EGTA和LaCl3能抑制Ca2+浓度升高. The Arabidopsis transformed aequorin was obtained by floral-dip method. The paper studied the concentration changes of cytoplasmic free calcium responses to low temperature. The results showed that cold shock could elicit an immediate rise in cytoplasmic free calcium concentration. EGTA and lanthanum caused a partial inhibition of cold shock Ca^2＋ elevation.
作者 张国增 张小莉
机构地区 河南大学生命科学学院植物逆境生物学重点实验室
出处 《河南大学学报（自然科学版）》 CAS 北大核心 2008年第6期609-612,共4页 Journal of Henan University:Natural Science
基金 海外青年学者合作研究基金资助(30428026)
关键词 水母发光蛋白 低温胁迫 CA^2+浓度 信号转导 拟南芥 aequorin cold stress Ca^2＋ concentration signal transduction Arabidopsis thaliana
分类号 Q945.78 [生物学—植物学]
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参考文献8

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同被引文献133

  • 1夏快飞,梁承邺,叶秀粦,徐信兰.不同类型水稻不育系药隔发育过程中Ca^(2+)的分布变化[J].西北植物学报,2005,25(8):1558-1565. 被引量：4
  • 2史娟,李继硕.细胞内钙成像和钙测定的基本原理及应用[J].神经解剖学杂志,2006,22(4):455-462. 被引量：9
  • 3王春雷,张绍铃.荧光标记在植物花粉管构造及生长特性研究中的应用[J].西北植物学报,2007,27(2):407-413. 被引量：15
  • 4TORRECILLA I,LEGANES F,BONILLA I,FERNANDEZ-PINAS F.Use of recombinant aequorin to study calcium homeostasis and monitor calcium transients in response to heat and cold shock in Cyanobacteria[J].Plant Physiol.,2000,123(1):161-176.
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  • 6KNIGHT H,TREWAVAS A J,KNIGHT M R.Calcium signaling in Arabidopsis thaliana responding to drought and salinity[J].Plant J.,1997,12(5):1 067-1 078.
  • 7KIM J C,LEE S H,CHEONG Y H,YOO C M,LEE S I,CHUN H J,YUN D J,HONG J C,LEE S Y,LIM C O,KNIGHT H,BRANDT S,KNIGHT M R.A history of stress alters drought calcium signalling pathways in Arabidopsis[J].Plant J.,1998,16(6):681-687.
  • 8PAULY N,KNIGHT M R,THULEAU P,LUIT A H,MOREAU M,TREWAVAS A J,RANJEVA R,MAZARS C.Control of free calcium in plant cell nuclei[J].Nature,2000,405(6788):754-755.
  • 9LOGAN D C,KNIGHT M R.Mitochondrial and cytosolic calcium dynamics are differentially regulated in plants[J].Plant Physiol.,2003,133(1):21-24.
  • 10BUTTON D,EIDSATH A.Aequorin targeted to the endoplasmic reticulum reveals heterogeneity in luminal Ca2+ concentration and reports agonist-or IP3-induced release of Ca2+[J].Mol.Biol.Cell.,1996,7(3):419-434.

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河南大学学报（自然科学版）
2008年 第6期

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