摘要
研究了稀酸预处理过的玉米芯酶水解过程中，木聚糖酶的补充对葡萄糖、木糖得率的影响。结果表明：玉米芯的酶水解过程中，添加适量的木聚糖酶，可提高葡萄糖和木糖的生成速度，但是，酶解24h之后，木聚糖酶的这种强化作用基本消失，此时葡萄糖和木糖生成速度基本上与初始酶用量无关。在总蛋白质含量不变的情况下，采用含等量蛋白质的纤维素酶和木聚糖酶所构成的混合酶系，明显地比单一等量蛋白质的纤维素酶提高了单位蛋白质的产糖率，有利于降低酶解成本。在纤维素酶量为5-35FPU／g（干原料）范围内，适宜的木聚糖酶添加量为60IU／g（干原料）；在其他条件相同情况下，分别采用35FPU／g（干原料）的纤维素酶和混合酶系[15FPU／g（干原料）的纤维素酶与60IU／g（干原料）木聚糖酶混合]时，72h时的糖得率（葡萄糖和木糖）几乎相等，因此，采用纤维素酶和少量的木聚糖酶的混合酶系可明显地降低纤维素酶的使用量，降低酶解成本。
The effect of supplementation of xylanases from the corncob pretreated by diluted sulfuric acid. tt was Trichoderma viride was investigated on the conversion of shown that addition of xylanases increased the initiat conversion rate of corncob into glucose and xyiose, but the enhancement was insignificant after 24h. It was demonstrated that conjugated action of cellulases and xylanases resulted in a higher final sugar production as compared to cellulases alone based on per gram of protein.It was shown that the optimal loading of xylanses was 60IU/g （O D substrate）when cellulase loading from 5 to 35 FPU/g was used during the hydrolysis of the pretreated corncob.The yields of glucose and xylose at 72h using the 35FPU/g cellulases were almost equal to those using a mixture of 15FPU/g cellulases and 60IU/g xylanases.Therefore,addition of xylanase would provide an opportunity to decrease cellulase and protein loading at the same hydrolysis extent.
出处
《食品工业科技》
CAS
CSCD
北大核心
2010年第11期228-231,共4页
Science and Technology of Food Industry
