摘要
本文采用ＤＮＡ琼脂糖凝胶电泳研究了２３５Ｕ诱发的免疫细胞（人淋巴细胞白血病细胞株Ｍｏｌｔ－４细胞和巨噬细胞株Ａｎａ－１细胞）凋亡，探讨了细胞因子ＩＬ－２和ＩＬ－６的防护作用。结果表明，经２３５Ｕ照射达６ｈ以上的各组Ｍｏｌｔ－４和Ａｎａ－１细胞，均可观察到呈现凋亡细胞特征的阶梯状条带形成；而预先使用细胞因子ＩＬ－２和ＩＬ－６，对２３５Ｕ诱发的免疫细胞ＤＮＡ链断裂有显著的防护作用。
In this paper, the 235 U induced apoptosis in immune cells(Molt 4 cell, a human acute lymphoblastic leukemia cell line, and Ana 1 cell, a macrophage cell line) was studied with the DNA agarose gel electrophoresis. At the same time, the protective effect of cytokines, IL 2 and IL 6, on the induction of apoptosis by 235 U in these cells was inquired. The results indicated that while Molt 4 and Ana 1 cells were exposed to 235 U more than 6 hours, the electrophoretogram of DNA for these cells showed a ladder pattern which is characteristic of apoptosis However, pretreatment of cells with IL 2 and IL 6 could reduce the induction of apoptosis by 235 U in both Molt 4 and Ana 1 cells. (
出处
《辐射防护》
CAS
CSCD
北大核心
1999年第3期179-182,194,共5页
Radiation Protection
基金
核工业科学基金
关键词
DNA凝胶电泳
免疫细胞凋亡
细胞凋亡
防护
铀235
DNA Gel Electrophoresis, Uranium 235, Immune Cell, Apoptosis, IL 2, IL 6, Radioprotection)
