摘要
背景:间充质干细胞主要存在于结缔组织和器官间质中,具有强大的增殖能力和多向分化潜能及免疫调节等多种功能;同时具有来源方便,易于分离、培养、扩增和纯化等优点,因而在组织工程,基因治疗等领域日益受到重视。目的:综述不同来源间充质干细胞的生物学特性、对其他细胞的支持作用、免疫原性、免疫调节作用及其应用。方法:应用计算机检索2001-03/2010-03 PubMed数据库相关文章,检索词为"mesenchymal stem cells,tissue engineering",同时检索万方数据库相关文章,检索词为"间充质干细胞,组织工程"。共检索到文献182篇。结果与结论:间充质干细胞来源于中胚层间充质,不仅具有多向分化的潜能,单独应用于组织工程,而且具有为其他细胞的生长繁殖提供支持的作用,可以调节机体的免疫状态等特性而被广泛应用于医学领域。目前,间充质干细胞功能的研究虽然取得了一些成就,但有关研究才刚刚起步,仍存在许多理论和技术问题,对于如何有效控制其在体内定向分化、增殖、迁移并整合参与宿主细胞组织功能还需要大量深入和系统研究。
BACKGROUND：Mesenchymal stem cells （MSCs） form connective tissue and organ interstitial in the body.MSCs are characterized by strong proliferative ability, multi-differentiation potential and immunomodulation.With advantages such as convenient source, easy to separate, culture, amplify and purificate, MSCs play an important role in tissue engineering and gene therapy research.OBJECTIVE：To review biological proterty of different sources of MSCs, support role for other cells, immunogenicity, immune regulation and their application.METHODS：The relevant article published between March 2001 and March 2010 were searched for in PubMed database by computer with the key wory ＂mesenchymal stem cells, tissue engineering＂ .Also searched related article in Wanfang Database with the key wory of ＂mesenchymal stem cells, tissue engineering＂.A total of 182 documents were retrieved.RESULTS AND CONCLUSION：MSCs are derived from embryonic mesoderm, have the potential of multi-directional differentiation, and can be applied to tissue engineering.Moreover, MSCs provide support for cell growth, regulate immune state of the body, and have been extensively utilized in medical field.At present, the studies of MSCs function have achieved some successes, but these studies are at early stage and there are many theoretical and technical problems.Further studies are needed to determine how to effectively control the MSCs directional differentiation, proliferation, migration and integration in vivo and how to participate in host cell tissue function.
出处
《中国组织工程研究与临床康复》
CAS
CSCD
北大核心
2010年第36期6800-6804,共5页
Journal of Clinical Rehabilitative Tissue Engineering Research
基金
国家自然科学基金(30960388)
云南科技厅联合专项基金资助项目(2007C0007R)
昆明医学院博士创新基金项目(KM2007D03)~~
