摘要
在ＮＢ培养基上，拮抗细菌Ｂ－９１６在３０℃～３３℃、ｐＨ７．０～８．５时，均能生长良好，培养３０～３６ｈ后，菌量可达８．２９×１０８ｃｆｕ／ｍｌ左右。初始接种量对Ｂ－９１６的生长有明显的影响。通过对１３种工业发酵用的培养基配方的筛选，结果表明，培养基Ｄ是发酵Ｂ－９１６的最佳培养基配方。用此配方进行工业发酵（３０℃，ｐＨ７．８～８．０发酵２６ｈ）菌量可达到３．
Strain B 916 ( Bacillus subtilis ) can grow vigorously in NB medium at 30℃～33℃,pH7 8 8 0,with a population of 8 29×10 8cfu/ml after 30-36 hours of culture.Initial inoculum dosage has different effect on the growth of B 916.Thirteen kinds of media commonly used in commerical production were screened.The medium of No.D was the best for the growth of B 916 with a maximum population of 3.84×10 10 11 cfu/ml when gowing at 30℃,pH7 8 8 0 for 26 hours of culture in fermentation tank
出处
《西南农业学报》
CSCD
北大核心
1999年第1期76-81,共6页
Southwest China Journal of Agricultural Sciences
基金
国际水稻研究所合作研究项目
关键词
水稻纹枯病
拮抗细菌
培养条件
发酵配方
rice sheath blight
antagonistic bacteria
culture condition
commercial production medium
