摘要
采用垂直平板聚丙烯酰胺凝胶电泳方法随机抽样测定了雪峰乌鸡和优黄２０００肉种鸡、ＩＳＡＢ－３８０父母代ＣＤ系母鸡血浆酯酶的遗传多态性，并借助所测位点基因频率估计了平均基因杂合度。结果表明：三种鸡血浆酯酶均存在遗传多态现象。优黄２０００肉种鸡和雪峰乌鸡Ｅｓ－１区有二条多态性酶带即Ａ带和Ｂ带，ＩＳＡＢ－３８０父母代ＣＤ系母鸡除Ａ和Ｂ带外还存在Ｃ带，但Ｃ带的频率很低。肉鸡和乌鸡Ｅｓ－ＩＢ频率大于Ｅｓ－１Ａ频率，而ＩＳＡＢ－３８０父母代ＣＤ系蛋鸡Ｅｓ－１Ａ频率大于Ｅｓ－ＩＢ频率。雪峰乌鸡位点平均基因杂合度明显低于优黄２０００肉种鸡和ＩＳＡＢ－３８０父母代鸡，可能是由于湘西地理封锁而长期封闭繁育所致。鉴于雪峰乌鸡已达到较高的品种内纯合度，今后育种应侧重经济性状的提高。
The polymorphism of plasma esterase was investigated with chickens randomly sampled froma breeding population of Xuefeng black-boned chickens,a Super-Y 2000 broiler breeding popution and a parental CD stock of ISA B-380 layers by using vertical discontinuous polyacrilamide gel electrophoresis.Freauencies of genes and genotypes were estimated for loci Es-1 and Es-2,Average heterozygosity was also calculated regarding these olci.The results indicated that polymorphism of plasma esterase existed in all of the 3 populations.Two bands(A and B)and three genotypes (AA ABand BB)were detected at locus Es-1 in super-Y 2000 broiler breeding stock and Xuefeng black-boned chickens;an addition C band was found at this locus in the parental CD population of ISA B-380 layers.Allelic frequency of Es-1B was higher than that of Es-1A in the first two populations ,while Es-1A was of higher frequency than Es-1B in the parental population of ISA B-380 layers .It was also noticed that the population of Xuefeng black-boned
出处
《特产研究》
1999年第1期13-16,共4页
Special Wild Economic Animal and Plant Research
基金
湖南省"九五"攻关重点资助
关键词
酯酶
遗传
多态性
基因杂合度
乌鸡
esterase
genetic polymorphism
heterozygosity
black-boned chickenschickens exhibited the least heterozygosity among the 3 chickens populations under,probably because of a long history of closed breeding resulting from gellogical isolation.
