摘要
目的观察雷公藤内酯醇(TP)对皮肤鳞癌A431细胞株增殖与凋亡、环氧合酶-2(COX-2)和生存素(survivin) mRNA表达水平的影响,探讨TP抗皮肤肿瘤的作用机制。方法以体外培养的人皮肤鳞癌A431细胞株为研究对象,MTT比色法测定不同浓度TP对A431细胞株增殖活性的影响,光镜观察细胞形态学变化,流式细胞仪检测TP作用于A431细胞株后细胞周期的分布以及凋亡峰的出现,RT-PCR法检测TP对A431细胞株COX-2和survivin mRNA表达水平的影响。结果随着TP剂量的增加及作用时间的延长,TP对A431细胞株增殖活性的抑制作用增强,TP能够诱导A431细胞株凋亡和改变细胞周期的分布,与对照组相比,G0/G1期细胞比例增多(P<0.05),S期细胞比例减少(P<0.05);另外,TP能抑制A431细胞COX-2和sur-vivin mRNA的表达,不同浓度处理组两两相比,差异具有显著性(P<0.01)。结论 TP通过诱导A431细胞凋亡、抑制A431细胞COX-2和survivin mRNA表达发挥抗皮肤肿瘤的作用。
ObjectiveTo investigate the effects of triptolide on the proliferation,apoptosis,cycloxygenase-2（COX-2） and survivin mRNA expression in skin squamous carcinoma cell line A431 and to explore its anti-humor mechanism.MethodsHuman skin squamous carcinoma cell line A431 cultured in vitro was treated with various doses of triptolide,cell proliferation activity was determined by MTT assay.Cell morphological changes were observed under light microscope.The cell cycle and apoptosis rate were assessed by flow cytometry.COX-2 and survivin mRNA expressions were measured by RT-PCR.ResultsTriptolide significantly inhibited the proliferation of A431 cells with a dose-and time-dependent manner.Triptolide also induced cell apoptosis and changed the distribution of cell cycle phase in A431 cells.Compared with the control group,the G0/G1 phase A431 cell rate increased and the rate of S phase cells decreased in TP-treated group（P〈0.05）.In addition,triptolide down-regulated COX-2 and survivin mRNA expression（P〈0.05）.ConclusionTP could play an anti-skin tumor role by inducing cell apoptosis and inhibiting COX-2 and survivin mRNA expressions in A431 cells.
出处
《中国现代医学杂志》
CAS
CSCD
北大核心
2010年第17期2587-2591,共5页
China Journal of Modern Medicine
