摘要
采用聚乙二醇(PEG)/(NH4)2SO4双水相体系分离纯化葡萄籽中的蛋白质.确定其双水相体系组成20%PEG600-20%(NH4)2SO4,通过单因素和星点设计探讨NaCl质量分数、pH值、温度、粗提液加入量对萃取效果的影响.结果表明:温度和蛋白质粗提液加入量对蛋白质萃取效果影响最大,其次为NaCl质量分数,pH的影响较小.最佳萃取条件是:NaCl质量分数0.06%、pH为7.05、温度25℃、蛋白质粗提液与双水相体系体积比为3.97∶10,在此条件下萃取率达77.52%,分配系数为4.61.
An aqueous two -phase system composed of 20% PEG600 and 20% （NH4 ）2SO4 was used to ex tract total protein from grape seeds. The effects of mass fraction of NaCI, pH value, extraction temperature and leaching solution volume on the extraction rates and distribution coefficient were explored by one - factor - at - a -time and central composite design （CCD）experiments. The results showed that：extraction temperature and leac- hing solution volume were major affecting factors, mass fraction of NaC1 and pH value exhibited less effect on the extraction efficiency of total protein . The best extraction condition were NaC1 mass fraction 0.06%, pH value 7.05 ,temperature 25℃ ,volume ratio of leaching solution to aqueous two - phase system was 3.97： 10.00. On the optimization condtition, the extraction rates of protein and distribution coefficient reached 77.52% and 4.61.
出处
《云南大学学报（自然科学版）》
CAS
CSCD
北大核心
2013年第6期824-832,共9页
Journal of Yunnan University(Natural Sciences Edition)
基金
河西学院大学生科技创新活动项目
关键词
双水相萃取
星点设计
葡萄籽蛋白质
aqueous two -phase extraction
central composite design
grape seeds protein
