摘要
目的探讨应用阿霉素制备兔扩张型心肌病(DCM)模型及兔骨髓间充质干细胞(MSCs)的分离培养方法 ,旨在为研究DCM治疗方法的选择以及临床疗效的观察奠定基础,为细胞移植的准备提供方法学依据。方法应用1周1次每次2 mg/kg,及每周2次每次1 mg/kg,均连续8周的给药方式进行兔DCM模型的制备,给药结束后3周处死动物,行心肌组织病理学检查比较其效果;分别应用密度梯度离心法及全骨髓培养法进行兔MSCs的体外分离培养,比较其生物学特性。结果心肌组织病理学检查结果显示,两组动物心肌均呈DCM样改变,模型制备效果一致,动物死亡率和体重减轻率无统计学差异。应用密度梯度离心法和全骨髓培养法均可获得MSCs,但所获细胞纯度及其生物学特性略有差异。结论本研究表明兔作为实验动物经济有效,两种给药方式模型制备效果一致,推荐使用1周1次的给药方式;两种培养方法均可获得MSCs细胞,可根据不同实验中具体需要的细胞数量和细胞纯度进行不同培养方法的选择。
Objective Establish Adriamycin-induced dilated cardiomyopathy（DCM） modle of rabbits through ear-border vein and explore the suitable culture condition of rabbit mesenchymal stem cells（MSCs） for researching the cell-transplantation therapy of DCM.Methods Establish the DCM modle by two ways of administer.One way is injecting adriamycin 2mg/kg body wt once per week for 8 weeks,and the other is injecting adriamycin 1mg/kg body wt twice a week for 8 weeks.We compare the efficiency of two methods by pathology result after 3 weeks.The whole medulloculture method and Ficoll-Hypaque density gradient solution method were used to isolate MSCs,and then observing the growth property.Results After 3 weeks of final injection,the histomorphology changes of myocardium coincided with the characteristics of DCM.Two ways of administering had the same efficiency.By the whole medulloculture method,cell number we got was greater than that through the Ficoll-Hypaque density gradient solution method,but the Purity was worse.Conclusion The DCM rabbit model which prepared by two ways of administering was similar to the histopathology changes of DCM in human and we suggest the way of administering by once a week.We can choose the method of culturing the MSCs by different demands of cell number and cell purity in different experiments.
出处
《中国实验诊断学》
北大核心
2010年第10期1539-1542,共4页
Chinese Journal of Laboratory Diagnosis
基金
哈尔滨市科技创新人才(优秀学科带头人)研究专项资金No.2006RFXXS030
教育部黑龙江省部共建心肌缺血机理与诊疗技术重点实验室
