摘要
目的 探讨 β 磷酸三钙 (β tricalciumphosphate ,β TCP)多孔陶瓷与骨髓间充质干细胞 (bonemarrowmes enchymalstemcells ,BMSCs)的生物相容性 ,为组织工程方法修复骨和软骨缺损提供依据。 方法 将骨髓间充质干细胞与β 磷酸三钙多孔陶瓷复合并体外培养 ,然后进行形态学、细胞增殖、DNA含量、蛋白质含量测定。 结果 骨髓间充质干细胞能够贴附在 β 磷酸三钙多孔陶瓷孔隙内生长 ,其DNA复制和蛋白合成功能不受 β 磷酸三钙的影响。 结论 β 磷酸三钙多孔陶瓷生物相容性良好 ,可作为组织工程的支架材料应用于骨和软骨缺损的修复。
Objective To study the biocompatibility of β-tricalcium phosphate (β-TCP) porous ceramic with co-cultured bone marrow mesenchymal stem cells (BMSCs) in vitro.Methods BMSCs were seeded into β-TCP and cultured in vitro, then their morphological characteristics, cell proliferation, DNA content, protein content were detected. Results BMSCs could attach to the inner surface of β-TCP, and their capability of DNA replication and protein production were not affected by β-TCP. Conclusion β-TCP has good biocompatibility and can provide suitable space for BMSCs. It can be used to repair bone or osteochondrol defects as scaffold in tissue engineering.
出处
《华中科技大学学报（医学版）》
CAS
CSCD
北大核心
2004年第5期615-618,共4页
Acta Medicinae Universitatis Scientiae et Technologiae Huazhong
基金
国家"8 6 3计划"资助项目 (No 2 0 0 1AA2 16 0 31)
