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基于核糖体28S rRNA对叶螨的鉴定及其系统发育分析 被引量:9

Identification and phylogenetic analysis of spider mites based on 28S rRNA sequences
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摘要 通过研究核糖体28SrRNA的D1~D2部分序列来阐述蜱螨亚纲叶螨科6个属11个物种的系统发生关系。研究结果证实了形态学上定义的叶螨属(Tetranychus)、全爪螨属(Panonychus)、双叶螨属(Amphitetranychus)、裂爪螨属(Schizotetranychus)、缺爪螨属(Aponychus)和岩螨属(Petrobia)等6个属分别形成一个单系,然而在叶螨属内截形叶螨(T.truncatus)、土耳其斯坦叶螨(T.turkestani)、神泽叶螨(T.kanzawai)、朱砂叶螨(T.cinnabarinus)和二斑叶螨(T.urticae),及全爪螨属内苹果全爪螨(Pa.ulmi)和柑橘全爪螨(Pa.citri)各物种的系统发生地位并没有被很好地确定。因此,基于核糖体28SrRNA序列无法将叶螨鉴定到种水平,但却为属水平上的鉴定提供了重要的参考。 Relationships of 11 species from six genera in the Tetranychidae in the Acari subclass were inferred from partial D1 D2 region of nuclear 28S rRNA sequences. The results confirm the monophyly of the morphologically defined Tetranychus,Panonychus,Amphitetranychus, Schizotetranychus, Aponychus and Petrobia. However, the phylogeny of T. truncatus, T. turkestani, T. kanzawai,T. cinnabarinus and T. urticae from Tetranychus,and Pa. ulmi and Pa. citri from Panonychus can not be resolved well. The study based on 28S rRNA sequences could not identify spider mites to species level,but provide a significant reference to the identification of different genera.
出处 《南京农业大学学报》 CAS CSCD 北大核心 2010年第5期49-54,共6页 Journal of Nanjing Agricultural University
基金 国家自然科学基金项目(30871635) 国家公益性农业行业科技项目(nyhyzx07057)
关键词 叶螨 物种鉴定 28SrRNA 系统发育分析 DNA条形码 spider mites species identification 28S rRNA phylogenetic analysis DNA barcoding
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  • 1Bolland H R, Gutierrez J, Flechtmann C H W. World Catalogue of the Spider Mite Family (Acari: Tetranychidae) [ M]. Leiden: Brill Academic Publishers, 1998:51 -498.
  • 2Navajas M, Lagnel J, Gutierrez J, et al. Species-wide homogeneity of nuclear ribosomal ITS2 sequences in the spider mite Tetranychus urticae contrasts with extensive mitochondrial COI polymotphism [J]. Heredity, 1998, 80:742 -752.
  • 3Dupont L M. On gene flow between Tetranychus urticae Koch, 1836 and Tetranychus cinnabarinus (Boisduval) Bondreaux, 1956 (Acari: Tetranyehidae) : synonymy between the two species [ J]. Entomol Exp Appl, 1979, 25:297 -303.
  • 4Zhang Z Q, Jacobson R J. Using adult female morphological characters for differentiating Tetranychus urticae complex ( Acari : Tetranychidae) from greenhouse tomato crops in UK [ J]. Syst Appl Acarol, 2000, 5:69 -76.
  • 5Navajas M, Boursot P. Nuclear ribosomal DNA monophyly versus mitochondrial DNA polyphyly in two closely related mite species : the influence of life history- and molecular drive [ J]. Proc R Soc Lond B, 2003, 270:S124 - S127.
  • 6Ros V I D, Breeuwer J A J. Spider mite (Acari: Tetranychidae) mitochondrial COl phylogeny reviewed: host plant relationships, phylogeography, reproductive parasites and barcoding [ J]. Exp Appl Acarol, 2007, 42:239 -262.
  • 7Hebert P D N, Cywinska A, Ball S L, et al. Biological identifications through DNA barcodes [J]. Proc R Soc Lond B, 2003, 270: 313 -322.
  • 8Hebert P D N, Ratnasingham S, de Waard J R. Barcoding animal life: cytochrome c oxidase subunit 1 divergences among closely related species [J]. Proc R Soc Lond B, 2003, 270:S96 -S99.
  • 9Ben-David T, Melamed S, Gerson U, et al. ITS2 sequences as barcodes for identifying and analyzing spider mites ( Acari : Tetranychidae) [J]. Exp Appl Acarol, 2007, 41 : 169 -181.
  • 10Navajas M, Lagnel J, Fauvel G, et al. Sequence variation of ribosomal internal transcribed spacers (ITS) in commercially important Phytoseiidae mites [J]. Exp Appl Acarol, 1999, 23:851 -859.

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