摘要
比较了琼脂斜面法、穿刺石蜡法和甘油冷冻法保存灿烂弧菌(Vibrio splendidus)和创伤弧菌(Vibrio vulnifi-cus)的效果。结果表明,甘油冷冻法和穿刺石蜡法的保存期显著比琼脂斜面法长,保存12月后,各保存方法细菌的毒力都有不同程度的下降,甘油冷冻法显著好于其它2种保存方法。
Three preservation methods （serially passage on bevel method, puncture-paraffin method, glycerol crypreservation method） for same strain of Vibrio splendidus and Vibrio vulnificus were compared in this paper. The result showed that the preserved stage of glycerol cry-preservation method and puncture-paraffin method was longer than anther method; the virulence of Listonella anguillarum preserving twelve months decreased in different degree for three preservation methods, glycerol cry-preservation method was better than the others.
出处
《水生态学杂志》
北大核心
2010年第5期136-140,共5页
Journal of Hydroecology
基金
浙江省科技厅科研院所专项公益技术攻关项目(2007F10009)
浙江省科技厅科研院所青年人才计划项目(2006R20001)
温州市科技兴海项目(S20070051)联合资助
关键词
灿烂弧菌
创伤弧菌
穿刺石蜡法
甘油冷冻法
Vibrio splendidus
Vibrio vulnificus
Puncture-paraffin method
Glycerol cry-preservation method
