摘要
目的研究高碳酸酸化预处理对脐静脉血管内皮细胞(EC)凋亡的影响。方法脐静脉EC培养传代后按处理方式不同分为6组(n=12):正常对照组(C组),缺氧复氧组(H/R组),缺氧预处理组(HPC组),30%CO2预处理内皮细胞10、30、60min组(10、30、60 min HCA组)。H/R组将细胞置于95%N2、5%CO2的缺氧培养箱中缺氧培养4 h,再在21%O2、5%CO2、74%N2的正常培养箱中复氧孵育24 h。HPC组将细胞置于缺氧培养箱中先行2个循环的缺氧10 min及正常培养箱中复氧10 min,其余处理方法同H/R组。10、30、60 min HCA组将细胞置于21%O2、30%CO2、49%N2培养箱中先行高碳酸酸化预处理10、30、60 min,其余处理方法同H/R组。C组将细胞置于正常培养箱至实验结束。用流式细胞仪测细胞凋亡指数。结果 HPC组,10、30、60 min HCA组凋亡指数小于H/R组,差异有统计学意义(P<0.01)。30 min HCA组的凋亡指数小于10 min HCA组,差异有统计学意义(P<0.01)。30 min HCA组的凋亡指数小于60 min HCA组,差异有统计学意义(P<0.05)。结论高碳酸酸化预处理可抑制离体EC的凋亡,30 min的抑制作用最强。
Objective To determine the the effect of hypercapnia preconditioning on the apoptosis of the umbilical endothelial cells.Methods Cultured Human umbilical vein cells were randomly divided into six groups（n=12）：control group（group C）,hypoxic and reoxygenation group（group H/R）,hppoxia preconditioning group（group HPC）,30%CO2 preconditioning for 10,30,60 minutes group（group 10,30,60 min HCA）.Cells in group H/R received hypoxia in the 95%N2,5%CO2 anerobic culturing box for 4 hours and reoxygenation in the 21%O2,5%CO2,74% N2 anerobic culturing box for 24 hours.Cells in HPC groups received 10 minutes hypoxia/10 minutes reoxygenation preconditioning that repeated for two times followed by long time hypoxia/rexygenation as in group H/R.Cells in HCA groups received 30% CO2 in 21%O2,30%CO2,49% N2 culturing box followed by the long time hypoxia/ rexygenation process as in group H/R.Cells in group C were cultured in the normal atmosphere until the test was over.Apoptosis index was obtained by the flow cytometry.Results The apoptosis index in the group HPC,10,30,60 min HCA were less than group H/R s（P〈0.01）.The apoptosis index in the group 30 min HCA was less than group 10 min HCA（P〈0.01）.The apoptosis index in the group 30 min HCA was less than group 60 min HCA（P〈0.05）.Conclusion Hypercarbonic acidosis preconditioning can prohibit the apoptosis of vascular endothelial cells and inhibition of 30 min Hypercarbonic acidosis preconditioning is most effective.
出处
《重庆医学》
CAS
CSCD
北大核心
2010年第20期2729-2731,2849,共4页
Chongqing medicine
基金
教育部博士点专项课题基金资助项目(20050533022)
关键词
高碳酸
预处理
血管内皮细胞
凋亡
hypercapnia
preconditioning
endothelial cells
apoptosis
