摘要
目的探讨口虾蛄乙酸乙酯提取物对鼻咽癌CNE-2Z细胞的增殖及凋亡相关蛋白表达的影响。方法用MTT比色法检测药物对细胞增殖的作用,流式细胞术和PI/Hoechst33258荧光双染法检测细胞凋亡,Western Blot法检测用药前后凋亡相关基因Bcl-2、Bax蛋白表达的改变。结果口虾蛄乙酸乙酯提取物对CNE-2Z细胞的增殖有显著抑制作用,且可诱导细胞凋亡;随着口虾蛄乙酸乙酯提取物浓度升高,Bax蛋白的表达逐渐增多,而Bcl-2蛋白表达改变不明显。结论口虾蛄乙酸乙酯提取物对CNE-2Z细胞有显著抗增殖及诱导凋亡作用,其诱导肿瘤细胞凋亡的可能机制与凋亡相关基因Bcl-2/Bax蛋白比值下降有关,但诱导凋亡不是主要机制。
Objective To explore the effect of Ethylacetate Extract from Squilla Oratoria on cell proliferation and apoptosis relative protein of nasopharyngeal cell line CNE-2Z.Methods The inhibitory rate of the proliferation was detected by MTT assay.Apoptosis was detected by PI/Hoechst3358 fluorescent staining and flow cytometry assay.The expression changes of relating-apoptosis gene proteins（Bcl-2,Bax） were detected by Western Blot assay.Results Ethylacetate extract of Squilla Oratoria can remarkably inhibit the proliferation of CNE-2Z cells.It was showed by using PI/Hoechst3358 fluorescent staining and flow cytometry assay that CNE-2Z cells were not only necrosis but also apoptosis after treated by Ethylacetate extract.The result of western blot showed that with the rising of ethylacetate extract concentration,the expression of bax increased gradually,but the Bcl-2 expression was unchanged.Conclusion Ethylacetate Extract from Squilla Oratoria can significantly inhibit the proliferation and induce apoptosis of CNE-2Z and its possible mechanism of inducing apoptosis might be related with the decline in Bcl-2/Bax,but not the dominant way.
出处
《重庆医学》
CAS
CSCD
北大核心
2010年第20期2710-2712,2849,共4页
Chongqing medicine
基金
广东省重点学科资助项目(GX9402)
东莞市高校科研科技计划资助项目(G200965)
关键词
口虾蛄
鼻咽癌
凋亡
CNE-2Z细胞
squilla oratoria
nasopharyngeal carcinoma
apoptosis
CNE-2Z cells
