摘要
为了从分子水平研究竹子光合作用的机理,采用RT-PCR及RACE方法从绿竹中分离了cab家族的一个基因,命名为BoLhca4-1(GenBank注册号为EF690303)。BoLhca4-1的cDNA序列全长931 bp,含有一个735 bp的开放阅读框,编码了一个244 aa的蛋白,分子量大小约为26.8 ku。序列分析结果表明,BoLhca4-1是光系统Ⅰ的一个捕光叶绿素复合蛋白基因,编码肽链与禾本科植物水稻的cab蛋白有着很高的同源性,达到86.1%。系统进化树分析表明,BoLhca4-1编码蛋白与水稻的cab蛋白亲缘关系较近。另外,对绿竹不同组织中BoLhca4-1基因的表达进行RT-PCR检测发现,其在叶片中的表达量较鞘和茎中要高。
To study the molecular mechanism of photosynthesis, the BoLhca4-1 （GenBank accession number： EF690303） , a member of the cab gent family, was isolated from Bambusa oldhamii by RT-PCR and RACE. The cDNA of BoLhca4-1 is 931 bp in length,which contains an 735 bp open reading frame （ORF） , encoding a polypeptide of 244 amino acids with predicted molecular mass of 26. 8 ku. The results of sequence analysis showed that the BoLhca4-1 is a cab binding protein gene of photosystem Ⅰ and its encoding protein is highly homologous to the cab protein from rice with 86. 1% identity. In addition, it was found that the Lhca4-1 was more related to the rice cab protein from the Phylogenetic tree. Based on the RT-PCR method, it was identified that BoLhca4-1 gene displays a higher expression level in leaf tissue than that in sheath and stem.
出处
《安徽农业大学学报》
CAS
CSCD
北大核心
2010年第4期643-648,共6页
Journal of Anhui Agricultural University
基金
林业公益性行业科研专项(200704001)
国家自然科学基金项目(30972328)
国际竹藤网络中心基本科研业务费重点项目(1632008002)共同资助
关键词
绿竹
光系统Ⅰ
捕光叶绿素a/b结合蛋白
基因克隆
表达分析
Bambusa oldhamii
photosystem Ⅰ
light harvesting chlorophyll a/b-binding protein （cab）
gene cloning
expression analysis
