摘要
捕光叶绿素a/b结合蛋白基因是绿色植物光合系统中的重要基因,其编码的蛋白与色素所形成的蛋白复合体在光化学反应、光保护等方面都起着重要的作用。采用RT-PCR技术和与RACE-PCR技术,从绿竹中克隆到捕光叶绿素a/b结合蛋白基因的全长cDNA编码区,命名为cab-BO2(GenBank登记号:EF088668)。该基因编码区长为792bp,编码263个氨基酸。通过加酶切位点的方法,将基因的编码区直接构建到载体pBI121多克隆位点。经过酶切检测,获得了包含35S启动子、cab基因、GUS报告基因和NOS终止区域的植物表达载体。
The cab gene coding light harvesting chlorophyll a/b-binding protein is one of key genes in photosynthesis system of green plants, which play an important role in light chemical reaction and light protection by binding with Chlorophyll a/b. A full-length cDNA coding region of cab gene was cloned from the first strand of bamboo cDNA by RT-PCR and RACE methods, which named as cab-BO2 （cab gene 2 from B. oldhamii） （GenBank accession No.： EF088668）. The coding region of cab-BO2 is 792 bp and encodes 263 amino acids. The cab-BO2 gene was cloned in the multiple cloning site of pBI121 vector directly by adding restriction enzyme sites. A plant expression vector containing 35S promoter, cab-BO2 gene, GUS and NOS region was confirmed by restriction enzyme analyses.
出处
《分子植物育种》
CAS
CSCD
2007年第3期431-435,共5页
Molecular Plant Breeding
基金
国家林业局948项目(2005-4-38
2004-4-58
2004-4-60)资助。
