摘要
目的观察葛芪达络汤对糖尿病周围神经病变(DPN)大鼠脑源性神经因子(BDNF)表达的干预作用,探讨葛芪达络汤防治DPN的可能机制。方法以链脲佐菌素(STZ)诱导糖尿病大鼠模型,中药汤剂灌胃,观测体质量、血糖、糖化血红蛋白的变化,坐骨神经的组织形态学改变。用免疫组化方法检测坐骨神经BNDF蛋白表达量。结果中药组大鼠坐骨神经病变比模型组减轻,轴突变性萎缩改变轻、髓鞘脱落较少,坐骨神经BDNF蛋白表达与模型组相比明显增多(P<0.05)。结论葛芪达络汤能上调DPN大鼠坐骨神经BNDF蛋白的表达,表明其对实验性DPN有一定的防治作用。
Objective It is to observe the intervention action of Geqidaluo decoction on the expression of brain derived neurotrophic factor（BDNF） in diabetic perineuropathy（DPN） rats and to approach the probable mechanism of Geqidaluo decoction preventing beforehand and curing DPN.Methods Diabetic rat models were induced with Streptozotocin（STZ）.Traditional Chinese medicine decoction was given intragastric administration.The changes of body mass,blood glucose,glycosylated hemoglobin and the histomorphology of sciatic nerve were observed.The expression of BDNF in sciatic nerve was detected with immunohistochemisty method.Results Compared with model group,the pathological changes of sciatic nerve in traditional Chinese medicine group was lightened,the change of axonal degeneration atrophy was lessened,the myelin sheath desquamation was fewer and the expression of BDNF in sciatic nerve was obviously increased（P 0.05）.Conclusion Geqidaluo decoction can up-regulate the expression of BDNF in sciatic nerve of DPN rats,which indicates that Geqidaluo decoction has determinate preventive and therapeutic effect on experimental DPN.
出处
《现代中西医结合杂志》
CAS
2010年第32期4121-4123,共3页
Modern Journal of Integrated Traditional Chinese and Western Medicine
关键词
葛芪达络汤
糖尿病周围神经病变
脑源性神经因子
Geqidaluo decoction
diabetic perineuropathy
brain derived neurotrophic factor
