摘要
检测香菇多糖分离纯化过程方法的准确度。取水提香菇多糖溶液,以其中多糖含量为基准,加0%、80%、100%和120%葡聚糖(465000),再进行醇沉、sevage法除蛋白、树脂脱色、过DEAE层析柱等操作,考察各个步骤的加样回收率。香菇多糖醇沉、除蛋白、脱色、过DEAE层析柱的平均加样回收率分别为89.76%、86.64%、95.39%和85.13%。本试验采用的分离提取方法的准确度高,操作简便易行。
To measure the accuracy of approach to separate and purificate the lentinan. Take appropriate water extraction of mushroom,among which taking polysaccharide content as a benchmark,each with 0 %, 80%, 100% and 120 % dextran （465 000）. Then depositing in alcohol,removing protein,decolor and passing the DEAE-Cellulose column. The recovery of each step was examined. The average recovery of the four steps were 89.76 %, 86.64 %,95.39 % and 85.13 %. The accuracy of the separation and extraction methods used in this study is good, and the operation is simple.
出处
《食品研究与开发》
CAS
北大核心
2010年第10期155-157,共3页
Food Research and Development
关键词
香菇多糖
分离纯化
加样回收率
lentinan
separate and purificate
the recovery
