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铅对胎鼠脑组织中蛋白激酶C和钙调蛋白M表达的影响 被引量：1

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摘要 目的 研究铅对不同发育时期胎鼠脑组织中蛋白激酶C（PKC）与钙调蛋白（CaM）mRNA和蛋白表达的影响.方法 孕鼠随机分为去离子水对照组、0.2%醋酸铅组和1.0%醋酸铅组,从妊娠第0天起开始通过自由饮水染铅.分别于孕12、18 d处死孕鼠,取胎鼠脑组织,原子吸收光谱法测定脑铅含量,反转录-聚合酶链反应（RT-PCR）和免疫印迹（Westen blotting）法分别观察各组PKC与CaM mRNA及蛋白的表达情况.结果 同一发育时期的染铅组胎鼠脑铅含量高于对照组,同一剂量染铅组孕18 d时胎鼠脑铅含量高于孕12 d时,差异均有统计学意义（P〈0.01,P〈0.05）.孕12 d时,与相应的对照组（mRNA：PKC：1.03±0.01、CaM：0.80±0.01,蛋白：PKC：0.70±0.05、CaM：0.75±0.05）相比,1.0%醋酸铅组胎鼠脑内PKC与CaM的mRNA（0.86±0.03、0.71±0.02）和蛋白表达（0.49±0.03、0.46±0.03）均降低;孕18 d时,0.2%和1.0%醋酸组胎鼠脑内PKC与CaM的mRNA和蛋白表达均较对照组降低,差异均有统计学意义（P〈0.05,P〈0.01）.结论 孕期母鼠染铅可使胎鼠脑组织铅含量升高,并引起PKC与CaM mRNA和蛋白表达水平的下降.
作者 管彤 邸金茹 商博东 王凤山
机构地区 天津市疾病预防控制中心毒理室
出处 《中华劳动卫生职业病杂志》 CAS CSCD 北大核心 2010年第9期693-695,共3页 Chinese Journal of Industrial Hygiene and Occupational Diseases
关键词 铅 蛋白激酶C 钙调蛋白
分类号 R686 [医药卫生—骨科学]
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参考文献9

  • 1Needleman H.Lead poisoning.Annu Rev Med,2004,55:209-222.
  • 2李红,马海燕,赵延祜.围产期铅毒性[J].中华围产医学杂志,2004,7(5):315-317. 被引量：13
  • 3Wu J,Rowan MJ,Anwyl R.Synaptically stimulated induction of group I metabotropic glutamate receptor-dependent long-term depression and depotentiation is inhibited by prior activation of metabotropic glutamate receptors and protein kinase C.Neuroscience,2004,123:507-514.
  • 4Lewit-Béntley A,Réty S.EF-hand calcium-binding proteins.Curr Opin Struct Biol,2000,10:637-643.
  • 5Liu Y,Buck DC,Macey TA,et al.Evidence that calmodulin binding to the dopamine D2 receptor enhances receptor signaling.J Recept Signal Transduct Res,2007,27:47-65.
  • 6Deng W,Poretz RD.Protein kinase C activation is required for the lead-induced inhibition of proliferation and differentiation of cultured oligodendroglial progenitor cells.Brain Res,2002,929:87-95.
  • 7Vetter SW,Leclerc E.Novel aspects of calmodulin target recognition and activation.Eur J Biochem,2003,270:404-414.
  • 8Sivagnanasundaram S,Fletcher D,Hubank M,et al.Differential gene expression in the hippocampus of the Dfl/+ mice:a model for 22q11.2 deletion syndrome and schizophrenia.Brain Res,2007,1139:48-59.
  • 9Opanashuk LA,Finkelstein JN.Induction of newly synthesized proteins in astroglial cells exposed to lead.Toxicol Appl Pharmacol,1995,131:21-30.

二级参考文献19

  • 1Hilliard A, Ramesh A, Zawia NH. Correlation between lead induced changes in cerebral ornithine decarboxylase and protein kinase C activities during development and in cultured PC 12 cells. Int J Dev Neurosci,1999,17:777-785.
  • 2Gulson BL, Jameson CW, Mahaffey KR, et al. Pregnancy increases mobilization of lead from maternal skeleton. J Lab Clin Med,1997,130:51-62.
  • 3Campagna D, Huel G, Girard F, et al. Environmental lead exposure and activity of delta-aminolevulinic acid dehydratase (ALA-D) in maternal and cord blood. Toxicology,1999,15,134:143-152.
  • 4Partl S, Herbst H, Schaeper F, et al. GFAP gene expression is altered in young rats following developmental low level lead exposure. Neurotoxicology, 1998,19:547-551.
  • 5Shannon M. Severe lead poisoning in pregnancy. Ambul Pediatr,2003,3:37-39.
  • 6Hertz-Picciotto I. The evidence that lead increases the risk for spontaneous abortion. Am J Ind Med, 2000,38:300-309.
  • 7Sowers M, Jannausch M, Scholl T, et al. Blood lead concentrations and pregnancy outcomes. Arch Environ Health, 2002,57:489-495.
  • 8Hernandez-Avila M, Peterson KE, Gonzalez-Cossio T, et al. Effect of maternal bone lead on length and head circumference of newborns and 1-month-old infants. Arch Environ Health,2002,57:482-488.
  • 9Mello CF, Kraemer CK, Filippin A, et al. Effect of lead acetate on neurobehavioral development of rats. Braz J Med Biol Res,1998,31:943-950.
  • 10Dietrich KN, Ris MD, Succop PA, et al. Early exposure to lead and juvenile delinquency. Neurotoxicol Teratol,2001,23:508-511.

共引文献12

同被引文献12

  • 1文涛,孙黎光,刘阳,赵晓光,宗志宏,高明奇,刘素媛.慢性铅暴露对小鼠CaMKII蛋白表达的影响[J].中国公共卫生,2006,22(10):1211-1212. 被引量：13
  • 2Suszkiw JB.Presynaptic disruption of transmitter release by lead.Neurotoxicology,2004,25:599-604.
  • 3Rizo J,Sudhof TC.C2-domains,structure and function of a universal Ca2+-binding domain.J Biol Chem,1998,273:15879-15882.
  • 4Wu J,Rowan MJ,Anwyl R.Synaptically stimulated induction of group i metabotropic glutamate receptor-dependent long-term depression and depotentiation is inhibited by prior activation of metabotropic glutamate receptors and protein kinase C.Neuroscience,2004 (123):507-514.
  • 5Nihei MK,McGlothan JL,Toscano CD,et al.Low level Pb2+ exposure affects hippocampal protein kinase Cγ gene and protein expression in rats.Neurosci Lett,2001,298:212-216.
  • 6Xu SZ,Shan CJ,Bullock L,et al.Pb2+ reduces PKCs and NF-kB in vitro.Cell Biol Toxicol,2006,22:189-198.
  • 7Cremin JD,Smith DR.In vitro vs in vivo Pb effects on brain protein kinase C activity.Environ Res,2002,90:191-199.
  • 8Deng W,Poretz RD.Protein kinase C activation is required for the lead-induced inhibition of proliferation and differentiation of cultured oligodendroglial progenitor cell.Brain Res,2002,929:87-95.
  • 9Xu SZ,Bullock E,Shan CJ,et al.PKC isoforms were reduced by lead in the developing rat brain.Int J Dev Neurosci,2005,23:53-64.
  • 10王凤山,刘忠慧,张静妹,邸金茹.铅对仔鼠海马蛋白激酶C和钙调蛋白基因表达与学习记忆的影响[J].中华劳动卫生职业病杂志,2008,26(3):139-142. 被引量：7

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中华劳动卫生职业病杂志
2010年 第9期

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