摘要
目的建立系统性表达Cramp转基因模型小鼠,为研究Cramp在衰老中的作用提供模型动物。方法把Cramp cDNA插入系统性表达CMV启动子下游,构建转基因表达载体,显微注射法建立Cramp转基因小鼠。PCR鉴定转基因小鼠的基因型,用RT-PCR和Western blotting方法筛选高表达品系。结果成功构建Cramp cDNA转基因载体,建立了Cramp转基因小鼠,通过RT-PCR和Western blotting方法筛选出3个高表达品系。结论建立了系统表达Cramp转基因小鼠,转入的Cramp基因在骨髓、脾脏、肝脏等组织高表达,为研究Cramp基因在衰老中的作用及机制提供了动物模型。
Objective To establish systemic expression Cramp transgenic mice,which can be used for the study of its function and effect on aging.Methods The construct was generated by inserting the murine Cramp cDNA into a vector with CMV promoter.The transgenic mice were created by the method of microinjection.The genotype of transgenic lines was identified by PCR and the expression level of the gene was determined by RT-PCR and Western blotting.Results Cramp cDNA transgenic vector was successfully constructed and Cramp transgenic mice were created.Three high expression lines were confirmed by RT-PCR and western blotting.Conclusion The systemic expression Cramp transgenic mouse was successfully established,and the Cramp gene was high expression in the tissue,such as bone marrow,spleen,and liver.The transgenic mouse could be a useful model for study its function in aging.
出处
《中国比较医学杂志》
CAS
2010年第9期12-15,共4页
Chinese Journal of Comparative Medicine
基金
科技重大专项重大新药创制项目[2009ZX09501-026]
关键词
Cramp
转基因小鼠
衰老
Cramp（cathelin-related antimicrobial peptide）
Transgenic mice
Aging
