摘要
目的:分析鉴定白喉乌头挥发油的组成组分,为其进一步的开发利用奠定基础。方法:取白喉乌头干粉250g,加蒸馏水1L,用电热套加热回流制备其挥发油,然后对挥发油直接进行GC-MS分析。色谱条件:RTX-5MS石英毛细管色谱柱(30m×0.32mm×1.0μm);升温程序:60℃保持1min,以10℃.min-1升至300℃,保持5min;载气(99.999%He),流速1.0mL.min-1,压力3.4kPa,进样量1μL;分流比30∶1。质谱条件:电子轰击(EI)离子源,电子能量70eV,传输线温度250℃,离子源温度200℃,质量扫描范围m/z30～500。采用Nist147谱库及Wiley7谱库进行检索。结果:分离出105个化合物,鉴定了其中的72个化合物,已鉴定出的化合物占总挥发油质量的86.81%。其中含量最高的成分为顺,顺-亚油酸(占29.48%)和n-棕榈酸(占21.08%);含量较高的成分有芳姜黄酮(4.78%)、十五烷酸(3.3%)、1,4-顺-1,7-反-菖蒲烯酮(2.85%)、2-羟基环十五烷酮(2.83%)、亚油酸甲酯(2.5%)、亚油酸乙酯(1.55%)和棕榈酸甲酯(1.41%)。结论:分析结果表明,白喉乌头挥发油中亚油酸和棕榈酸含量比较高,二者含量超过50%,具有一定的开发应用价值。
Objective：To analyze and identify the ingredients of volatile oil from Aconitum leucostomum Worosch.for its further exploitative use. Methods：250 g of Aconitum leucostomum dry powder and 1 L distilled water were mixed,and the volatile oil was extracted by refluxing through electric heating jacket,and then analyzed by GC-MS.Chromatographic conditions：RTX-5MS quartz capillary column（30 m×0.32 mm×1.0 μm）;temperature program：maintain in 60 ℃ about 1 min,elevated by 10 ℃·min-1 to 300 ℃,remain 5 min;carrier gas（99.999% He）,velocity 1.0 mL·min-1,pressure 3.4 kPa,the injection volume 1 μL;split ratio 30：1.MS conditions：electron impact（EI）ion source,electron energy 70 eV,transmission line temperature 250 ℃,ion source temperature 200 ℃,mass scan range m/z 30-500.And the resultes were contrasted with Nist 147 spectral library and Wiley 7spectral library. Results：105 compounds were separated,and 72 compounds were identified from the volatile oil and cis,cis-linoleic acid（accounts for 29.48%）,n-hexadecanoic acid（accounts for 21.08%）are major ingredients.Furthermore,the ingredients with more contents are ar-turmerone（4.78%）,pentadecanoic acid（3.3%）,1,4-cis-1,7-trans-acorenone（2.85%）,2-hydroxy-cyclopentadecanone（2.83%）,methyl linoleate（2.5%）,linoleic acid ethyl ester（1.55%）,methyl palmitate（1.41%）.Conclusion：Linoleic acid and hexadecanoic acid have higher contents and overall contents of both of them are more than 50%,and they have certain exploitive and practical value.
出处
《药物分析杂志》
CAS
CSCD
北大核心
2010年第9期1756-1759,共4页
Chinese Journal of Pharmaceutical Analysis
关键词
白喉乌头
GC-MS
挥发油
Aconitum leucostomum Worosch.
GC-MS
volatile oil
