摘要
目的:探讨线粒体融合基因2(Mfn2)在肝癌患者中的杂合性缺失(LOH)特点及其临床意义。方法:选用位于Mfn2附近的4个多态性微卫星标记,对29例肝癌患者进行LOH分析。结果:微卫星D1S2667、D1S2740、D1S434和D1S228的LOH发生率分别为21%、23%、21%和22%。Mfn2基因LOH患者的HBV感染,与患者年龄、肿瘤大小、包膜的完整性及分化程度存在一定的相关性(P<0.05);与患者性别、HCV感染、有无癌栓、肝内肿瘤的多发性、有无肝硬化以及AFP值高低无明显相关(P>0.05)。结论:肝癌患者Mfn2基因发生LOH与临床病理参数有一定的相关性,其在肝癌的发生和发展过程中起到一定作用。
Objective： To investigate the loss of heterozygosity（LOH） at mitofusin-2（Mfn2） gene in hepatocellular carcinoma（HCC） and its clinicopathological significance.Methods： Four high polymorphic microsatellite markers flanking Mfn2 were selected for LOH analysis in 29 cases of HCC.Results： The frequencies of LOH on D1S2667,D1S2740,D1S434 and D1S228 were 21%,23%,21% and 22%,respectively.LOH at Mfn2 was closely correlated with tumor size,age,capsule,differentiation and t HBV infection（P0.05）,not with gender,thrombosis,cirrhosis and serum AFP levels（P0.05）.Conclusion： LOH at Mfn2 gene in HCC is associated with the clinicopathological features of patients.
出处
《浙江大学学报（医学版）》
CAS
CSCD
北大核心
2010年第5期506-510,共5页
Journal of Zhejiang University(Medical Sciences)
基金
浙江大学基本科研业务费专项资金项目(KYJD090223)
关键词
癌
肝细胞
杂合子丢失
线粒体蛋白质类
基因缺失
Carcinoma
hepatocellular
Loss of heterozygosity
Mitochondrial proteins
Gene deletion
