摘要
目的观察纺锤体检查点蛋白BubR1在人类肝癌组织、正常肝组织以及肝癌细胞系(HepG2)、正常肝细胞系(LO2)有丝分裂期表达水平的差异,以探讨BubR1蛋白在肝癌发生中的作用。方法用荧光定量PCR和Western blot检测BubR1基因在肝癌组织、正常肝组织,以及在2种细胞中用Nocodazole处理前后的mRNA和蛋白的表达水平。结果BubR1基因在正常组织中的表达高于肝癌组织,在Nocodazole处理前2种细胞中表达无显著差异,在处理后2种细胞中表达均增高,但在LO2细胞中上调水平大于HepG2细胞。结论BubR1基因低表达可能在肝癌的发生过程中起重要作用。
Objective To study the differential expression of the spindle checkpoint protein BubR1 gene between human hepatoma tissue and human hepatic tissue, and between human hepatoma carcinoma cell line HepG2 and human hepatic normal cell line LO2 during mitotic period. Methods Nocodazole was employed to synchronize their cell phase at dividing phase. Real-time fluorescent-quantitation PCR and Western blotting were respectively employed to detect the expressions of BubRl mRNA and protein. Results The expressions of BubR1 mRNA and protein in human hepatic tissue was higher than that in human hepatoma tissue. The expressions of BubRl mRNA and protein showed no significant difference between HepG2 and LO2 cell lines before Nocodazole treatment. After being treated with Nocodazole, the expressions of BubR1 mRNA and protein in both cell lines were significantly increased and the increment in LO2 cells was higher than that in HepG2 cells. Conclusion Low expression of BubR1 gene is likely to play an important role in the genesis and development of human primary hepatocellular carcinoma.
出处
《第三军医大学学报》
CAS
CSCD
北大核心
2008年第22期2075-2077,共3页
Journal of Third Military Medical University
基金
国家自然科学基金(30371485)~~
