摘要
通过正交试验对东亚小花蝽Orius sauteri人工饲料微胶囊进行了配方优化研究,明确了海藻酸钠及壳聚糖的填充比例和芯壁比例在微胶囊产率、包埋率和含水率等方面的影响。各配方因素对产率的影响程度依次为:芯壁比>海藻酸钠填充比>壳聚糖填充比;而各因素对包埋率的影响程度依次为:壳聚糖填充比>海藻酸钠填充比>芯壁比;含水率受海藻酸钠填充比影响最大,而后依次为壳聚糖填充比和芯壁比;在海藻酸钠填充比为2.0%、壳聚糖填充比为0.6%及芯壁比为1∶3时,所得人工饲料微胶囊感官评定得分最高。以包埋率作为基准考量下,取最佳配比为海藻酸钠填充比为1.0%,壳聚糖填充比为0.8%,芯壁比为1∶1的配方,制作人工饲料微胶囊饲喂东亚小花蝽,与取食朱砂叶螨Tetranychus cinnabarinus组为对照,进行了饲喂效果试验。结果表明:与对照组相比,取食人工饲料微胶囊后,东亚小花蝽1、2龄若虫发育历期显著延长,雌成虫寿命显著缩短;羽化率和产卵前期差异不显著,但产卵期、产卵量和卵孵化率显著降低。此外,取食人工饲料微胶囊的东亚小花蝽成虫呼吸墒显著低于对照,运动速率则显著高于对照。
Orius sauteri is an important biological control agent and the massive factory production breeding of this agent is the core point in application processing. Based on the original artificial diet prescription,our present experiments promoted the artificial diet model for O. sauteri by microencapsulation technology. We confirmed the influences of sodium alginate,chitosan and the proportion of core material to wall-forming material on the yield rate,embedding rate,moisture rate and sensory evaluation level of microencapsulated artificial diet by orthogonal experiments. The results indicated that the influential order of ingredients to the yield rate successively was the proportion of core material to wall-forming material sodium alginate chitosan. The influential order of ingredients to embedding rate,however,was chitosan sodium alginate proportion of core material to wall-forming material. Furthermore,the sodium alginate influenced the moisture rate most and then chitosan. We observed similar tendency in sensory evaluation,and the corresponding optimum prescription was sodium alginate 2.0%,chitosan 0.6% and the proportion of core material to wall-forming material 1：3. Based on the embedding rate,we set the optimum prescription of microencapsulation as sodium alginate 1.0%,chitosan 0.8% and the proportion of core material to wall-forming material 1：1,and a diet of this prescription was used to compare the development status between the O. sauteri fed on the optimum artificial microcapsulated ated and CK （fed on Tetranychus cinnabarinus）. The results showed that there were significant differences in 1st and 2nd instar developmental duration. O. sauteri female adults showed significantly shorter life span on artificial diet than on CK. However,there was no significant difference in the emergence rate between two treatments. O. sauteri showed significantly lower oviposition duration,fecundity and hatch rate when feeding on artificial diet microcapsules although we did not observe any difference in pre-oviposition period at all. The intensive analysis of artificial diet effects showed that the respiratory quotient of O. sauteri fed on artificial diet was significantly lower than that on CK. However we observed a converse tendency in comparison of average creeping speed.
出处
《昆虫学报》
CAS
CSCD
北大核心
2010年第8期891-900,共10页
Acta Entomologica Sinica
基金
国家"973"计划项目(2009CB119206)
国家科技支撑计划项目(2006BAD08A02
2008BADA5B02)
北京市自然科学基金重点项目(6081002)
公益性行业(农业)科研专项(200803006)
关键词
东亚小花蝽
人工饲料
微胶囊
发育历期
繁殖力
Orius sauteri
artificial diet
micro-capsulation
developmental duration
fecundity
