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锁阳原花青素的HPLC-MS分析 被引量：13

HPLC-MS analysis of cynomorium songaricum rupr.
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摘要 采用反相液相色谱-电喷雾质谱(RP-HPLC/ESI-MS)分析法对锁阳提取液中原花青素的组成进行了分析。建立了采用RP-HPLC/ESI-MS对锁阳提取物中原花青素低聚体进行分析的方法。通过ESI-得到准分子离子[M-H]检测其单体和低聚体。研究结果发现:提取液中可检测出原花青素,初步检测出2个单体,6个二聚体,5个三聚体,但未检测出没食子酸单酯及其聚合体。建立的本方法不仅可以定性检测锁阳中含原花青素,且为非酯性原花青素。 The procyanidins in cynomorium extract are systematically analyzed by reversed phase liquid chromatography-electrospray mass spectrometry （RP-HPLC/ESI-MS）. Procyanidin monomer and oligomers are identified by corresponding molecular ions [ M - H ] by ESI- ionization methods. The results show that two proanthocyanidin monomers, six proanthocyanidin dimers, five proanthocyanidins trimers are detected in cynomofium extract, while gallic acid ester and its oolymers are not found. The results indicate that procyanidins can be detected in cynomorium, and the corresponding procyanidins are non-ester procyanidins.
作者 高乃群 曹玉华 陶冠军
机构地区 江南大学化工与材料工程学院 江南大学分析测试中心江苏
出处 《现代化工》 CAS CSCD 北大核心 2010年第10期91-93,共3页 Modern Chemical Industry
关键词 锁阳 原花青素 液相色谱 电喷雾质谱 cynomorium songaricum rupr. procyanidins HPLC ESI-MS
分类号 R284 [医药卫生—中药学]
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参考文献11

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二级参考文献12

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现代化工
2010年 第10期

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