摘要
为观察胰岛素样生长因子１受体基因在动脉粥样硬化组织中的表达及分布，建立实验性动脉粥样硬化家兔模型。采用人胰岛素样生长因子１受体ｃＲＮＡ探针进行组织原位杂交。结果发现，正常对照的家兔主动脉组织，仅在外膜显示有胰岛素样生长因子１受体ｍＲＮＡ的阳性表达，中膜及内膜均呈阴性；实验组主动脉的整个血管壁，包括外膜、中膜、新生内膜及动脉粥样硬化斑块组织均有胰岛素样生长因子１受体基因的表达。研究提示，增殖的血管平滑肌细胞、新生的内皮细胞及构成动脉粥样硬化斑块主要成分的泡沫细胞均为胰岛素样生长因子１受体ｍＲＮＡ表达的靶细胞，证实胰岛素样生长因子１受体基因参与动脉粥样硬化的发生。
Aim To determine the expression of insulin like growth factor 1 receptor (IGF 1R)gene in atherosclerotic plaques. Methods White rabbits were fed with either regular feed or high cholesterol diets for 12 weeks. By in situ hybridization we detected the expression of IGF 1R mRNA in rabbit atherosclerotic plaques and normal artery wall. Results Control rabbits, hybridization with antisense RNA probe only localized IGF 1R mRNA to adventitia. In atherosclerotic aortas, the positive signals were widely distributed in the vessel wall, including neointima, media, adventitia and atherosclerotic plaques. Conclusion Smooth muscle cells, endothelial cells and foam cells are the major source of IGF 1 receptor mRNA in atherosclerotic lesions. Over expression of IGF 1R mRNA might be related to the genesis of the atheromatous plaque.
出处
《中国动脉硬化杂志》
CAS
CSCD
1999年第2期103-105,共3页
Chinese Journal of Arteriosclerosis
基金
国家自然科学基金
关键词
胰岛素样
生长因子-1
原位杂交
动脉粥样硬化
Insulin Like Growth Factor 1
Receptor
mRNA Gene
In Situ Hybridization
Smooth Muscle Cell
Atherosclerosis
Rabbit
