摘要
研究了一种新型的流加方法──恒pH流加葡萄糖法，用于培养重组大肠杆菌生产人肿瘤坏死因子-α。流加后培养液中菌体OD(600)达到9．0，是在LB培养基培养的15倍，而α-肿瘤坏死因子的比活保持（1．05±0．11）×105u/mg，并建立了菌体生长的动力学方程。
A novel pH - stat fed - batch culture was studied here and applicated in culttire in Es-cherichia coli for human tumor necrosis factora. The cell densty reached 9. 0 OD600 andspecific activity rnaintained 1.05(0. 11 ×105u/mg prorein, while the Cell density was only 0.6OD600. The fermentation was described by kinetic equation.
出处
《微生物学杂志》
CAS
CSCD
1999年第1期46-49,共4页
Journal of Microbiology
关键词
肿瘤坏死因子
大肠杆菌
恒pH流加
动力学方程
Human tumor necrosis factor-α
Recombinant Escherichia coli
pH-stat fed-batch
kinetic equation
