摘要
应用一种新型的ｐＨ电极流加葡萄糖方法，用于培养重组大肠杆菌生产人α肿瘤坏死因子。流加后培养液中菌体ＯＤ６００达到９０，而α肿瘤坏死因子的比活保持１０５±０１１×１０５ｕ／ｍｇ。与指数流加方法比较，二者能达到的最大菌体密度相近，而利用ｐＨ电极流加葡萄糖更具有设备简单，操作方便的特点。
A novel fed batch culture using pH electrode was studied here and applicated in culture in Escherichia coli for human tumor necrosis factor α.The cell density reached 9.0 OD 600 while specific activity maintained 1.05±0.11×10 5 u/mg protein.Compared with exponential fed batch this novel fed batch method had the advantages of less instruments and easy to operate,while maintained similar cell density.
出处
《生物工程学报》
CAS
CSCD
北大核心
1999年第1期87-92,共6页
Chinese Journal of Biotechnology
